{"id":"8983cf95-7a57-56ee-ae59-02979363095a","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq9-assistance","predicate":"increases_efficiency_of","statement":"Adding COQ9 increased COQ7 catalytic efficiency about 1.5-fold in the reconstructed system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c11ec140-ff15-58b2-b759-0079f8a82282","mechanism_event_label":"A helper protein improved the neighboring enzyme reaction.","subject":{"id":"4e361c15-447f-5147-84fa-4e59a6faecd1","slug":"tet-coq9","display_name":"Reconstructed ancestral tetrapod COQ9","entity_type_key":"protein"},"object":{"id":"54ac8659-e9cf-50cc-a863-9aeabddbb7fe","slug":"tet-coq7","display_name":"Reconstructed ancestral tetrapod COQ7","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c11ec140-ff15-58b2-b759-0079f8a82282","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq9-assistance-event","event_type":"biochemical_relationship","label":"A helper protein improved the neighboring enzyme reaction.","description":"Adding COQ9 increased COQ7 catalytic efficiency about 1.5-fold in the reconstructed system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4e361c15-447f-5147-84fa-4e59a6faecd1","slug":"tet-coq9","display_name":"Reconstructed ancestral tetrapod COQ9","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"54ac8659-e9cf-50cc-a863-9aeabddbb7fe","slug":"tet-coq7","display_name":"Reconstructed ancestral tetrapod COQ7","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 23488, \"end_char\": 27391, \"text_sha256\": \"7147c9d517477cd7bbec4a155e5f13bc733d294380975977eba11b64517047f3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified reconstructed COQ metabolon with short-chain substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzyme combinations, methyl donors, reductants and metal additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Reconstructed ancestral tetrapod proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A helper protein improved the neighboring enzyme reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a0b9f62a-6ae7-5ee7-a9a7-2674d00467c6","evidence_kind":"source_excerpt","locator":"Lines 736-747","start_line":736,"end_line":747,"excerpt":"### coq10-coq9-assistance\nAdding COQ9 increased COQ7 catalytic efficiency about 1.5-fold in the reconstructed system.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A helper protein improved the neighboring enzyme reaction.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 23488, \"end_char\": 27391, \"text_sha256\": \"7147c9d517477cd7bbec4a155e5f13bc733d294380975977eba11b64517047f3\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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