{"id":"89746140-5ec3-52f6-b304-111ff3d473e3","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-irbp-retinol-release","predicate":"increases","statement":"IRBP at at least 10 micromolar increased all-trans-retinol release from bleached isolated toad retina during dark incubation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"44cc8b90-f89a-55ea-a2cc-787725c5ae3f","mechanism_event_label":"Extracellular IRBP helps remove retinol from the bleached retina.","subject":{"id":"5c834b93-4c9b-53ad-b61e-9f4f5bac0204","slug":"rbp3","display_name":"Interphotoreceptor retinoid-binding protein / IRBP","entity_type_key":"protein"},"object":{"id":"3aea1168-6a62-5cc7-9cad-45a77669f13a","slug":"retinal-retinol-release","display_name":"Retinal all-trans-retinol release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"44cc8b90-f89a-55ea-a2cc-787725c5ae3f","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-irbp-retinol-release-event","event_type":"biochemical_relationship","label":"Extracellular IRBP helps remove retinol from the bleached retina.","description":"IRBP at at least 10 micromolar increased all-trans-retinol release from bleached isolated toad retina during dark incubation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"transported molecule","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5c834b93-4c9b-53ad-b61e-9f4f5bac0204","slug":"rbp3","display_name":"Interphotoreceptor retinoid-binding protein / IRBP","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3aea1168-6a62-5cc7-9cad-45a77669f13a","slug":"retinal-retinol-release","display_name":"Retinal all-trans-retinol release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"2-90 micromolar IRBP; 5-180-minute incubation; HPLC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Measures release, not the entire in vivo round trip to RPE.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Bufo marinus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Extracellular IRBP helps remove retinol from the bleached retina.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[qtaishat-2005] Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination (2005). https://pubmed.ncbi.nlm.nih.gov/15935345/ DOI: 10.1016/j.exer.2005.03.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Isolated retina and extracellular medium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_direction","value_text":"retina to extracellular medium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b866791d-0d09-5c85-896d-8e7594784244","evidence_kind":"source_excerpt","locator":"Lines 789-799","start_line":789,"end_line":799,"excerpt":"### a-vision-irbp-retinol-release\nIRBP at at least 10 micromolar increased all-trans-retinol release from bleached isolated toad retina during dark incubation.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Extracellular IRBP helps remove retinol from the bleached retina.\norganism: Bufo marinus\ntissue_or_cell_type: Isolated retina and extracellular medium\nexperimental_model: 2-90 micromolar IRBP; 5-180-minute incubation; HPLC\nlimitations: Measures release, not the entire in vivo round trip to RPE.\ntransport_direction: retina to extracellular medium\n[qtaishat-2005] Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination (2005). https://pubmed.ncbi.nlm.nih.gov/15935345/ DOI: 10.1016/j.exer.2005.03.005","model_system":"2-90 micromolar IRBP; 5-180-minute incubation; HPLC","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [qtaishat-2005] Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination (2005). https://pubmed.ncbi.nlm.nih.gov/15935345/ DOI: 10.1016/j.exer.2005.03.005","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}