{"id":"89486cae-5f95-5c72-b0ec-28469b3c01bc","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-rarb-saa3-promoter","predicate":"activates-transcription-of","statement":"RAR beta occupied the mouse Saa3 promoter; mutation of RARE -224 abolished reporter activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a4e849b9-0595-558f-8046-66ca38decaf9","mechanism_event_label":"Epithelial retinoid sensing directly controls this mouse immune gene.","subject":{"id":"1ab58d8f-c076-5814-ac44-1492046497ae","slug":"rarb","display_name":"Retinoic acid receptor beta","entity_type_key":"protein"},"object":{"id":"383554ea-b4b0-5cb3-822a-e89d69176a10","slug":"mouse-saa3-gene","display_name":"Mouse Saa3 gene","entity_type_key":"gene"},"evidence_count":1,"mechanism_event":{"id":"a4e849b9-0595-558f-8046-66ca38decaf9","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-rarb-saa3-promoter-event","event_type":"biochemical_relationship","label":"Epithelial retinoid sensing directly controls this mouse immune gene.","description":"RAR beta occupied the mouse Saa3 promoter; mutation of RARE -224 abolished reporter activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce392c9e-6483-5914-b18b-d6a2520dbbf6","slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"},"role":"nutrient-context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"added-precursor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1ab58d8f-c076-5814-ac44-1492046497ae","slug":"rarb","display_name":"Retinoic acid receptor beta","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"383554ea-b4b0-5cb3-822a-e89d69176a10","slug":"mouse-saa3-gene","display_name":"Mouse Saa3 gene","entity_type_key":"gene"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_locator","value_text":"Figure 2; Methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse MODE-K cells and intestinal epithelial Rarb deletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 nM retinol plus 100 ng/mL LPS for 24 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct binding to Saa1/Saa2 was predicted, not demonstrated here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Epithelial retinoid sensing directly controls this mouse immune gene.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-gattu-2019] Epithelial retinoic acid receptor beta regulates serum amyloid A expression and vitamin A-dependent intestinal immunity (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6561173/ DOI: 10.1073/pnas.1812069116","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"MODE-K intestinal epithelial cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9c101ff4-8276-5578-b99d-f0b3a7544815","evidence_kind":"source_excerpt","locator":"Lines 1318-1329","start_line":1318,"end_line":1329,"excerpt":"### va-sig-rarb-saa3-promoter\nRAR beta occupied the mouse Saa3 promoter; mutation of RARE -224 abolished reporter activity.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Epithelial retinoid sensing directly controls this mouse immune gene.\norganism: Mus musculus\ntissue_or_cell_type: MODE-K intestinal epithelial cells\nexperimental_model: Mouse MODE-K cells and intestinal epithelial Rarb deletion.\nlimitations: Direct binding to Saa1/Saa2 was predicted, not demonstrated here.\nevidence_locator: Figure 2; Methods\nexposure: 100 nM retinol plus 100 ng/mL LPS for 24 h\n[va-gattu-2019] Epithelial retinoic acid receptor beta regulates serum amyloid A expression and vitamin A-dependent intestinal immunity (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6561173/ DOI: 10.1073/pnas.1812069116","model_system":"Mouse MODE-K cells and intestinal epithelial Rarb deletion.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-gattu-2019] Epithelial retinoic acid receptor beta regulates serum amyloid A expression and vitamin A-dependent intestinal immunity (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6561173/ DOI: 10.1073/pnas.1812069116","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}