{"id":"89019ddc-65ee-5dc5-a6c8-69d558ca7404","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-hacl2-er-localization","predicate":"localizes-to","statement":"Tagged human HACL2 colocalized with an ER marker and partitioned into the membrane fraction in HeLa cells.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"6dc2baab-561c-5036-92fa-83e4e0d506e3","mechanism_event_label":"This B1-dependent lipid-cleavage protein is associated with ER membranes; its location differs from peroxisomal HACL1.","subject":{"id":"e0cf5450-5814-51ea-bd5d-36026a3922f8","slug":"hacl2","display_name":"Human 2-hydroxyacyl-CoA lyase 2 / HACL2","entity_type_key":"protein"},"object":{"id":"ea69f7d2-43d8-5b62-8ed6-60d6ad580813","slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"6dc2baab-561c-5036-92fa-83e4e0d506e3","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-hacl2-er-localization-event","event_type":"biochemical_relationship","label":"This B1-dependent lipid-cleavage protein is associated with ER membranes; its location differs from peroxisomal HACL1.","description":"Tagged human HACL2 colocalized with an ER marker and partitioned into the membrane fraction in HeLa cells.","status":"provisional","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"e0cf5450-5814-51ea-bd5d-36026a3922f8","slug":"hacl2","display_name":"Human 2-hydroxyacyl-CoA lyase 2 / HACL2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ea69f7d2-43d8-5b62-8ed6-60d6ad580813","slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence","value_text":"[{\"paper_key\": \"kitamura-2017-hacl2\", \"source_bundle\": \"artifacts/thiamine_metabolism_sources.json\", \"passage_ids\": [\"p-47\", \"p-48\"], \"locator\": \"HACL2 is an ER membrane protein\", \"preservation\": \"Exact text retained in the source bundle; full source document retained when openly retrievable.\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HACL2 immunofluorescence and fractionation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tagged overexpression localization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Thiamine (vitamin B1)","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This B1-dependent lipid-cleavage protein is associated with ER membranes; its location differs from peroxisomal HACL1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[kitamura-2017-hacl2] Phytosphingosine degradation pathway includes fatty acid α-oxidation reactions in the endoplasmic reticulum (2017). https://pubmed.ncbi.nlm.nih.gov/28289220/ DOI: 10.1073/pnas.1700138114","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Transfected HeLa cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7370f5c7-ec81-5481-9c2d-f64b6d32aa9b","evidence_kind":"source_excerpt","locator":"Lines 1031-1042","start_line":1031,"end_line":1042,"excerpt":"### b1-hacl2-er-localization\nTagged human HACL2 colocalized with an ER marker and partitioned into the membrane fraction in HeLa cells.\nCondition category: normal\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This B1-dependent lipid-cleavage protein is associated with ER membranes; its location differs from peroxisomal HACL1.\norganism: Homo sapiens\ntissue_or_cell_type: Transfected HeLa cells\nexperimental_model: Human HACL2 immunofluorescence and fractionation.\nlimitations: Tagged overexpression localization.\nevidence: [{\"paper_key\": \"kitamura-2017-hacl2\", \"source_bundle\": \"artifacts/thiamine_metabolism_sources.json\", \"passage_ids\": [\"p-47\", \"p-48\"], \"locator\": \"HACL2 is an ER membrane protein\", \"preservation\": \"Exact text retained in the source bundle; full source document retained when openly retrievable.\"}]\nnutrient: Thiamine (vitamin B1)\n[kitamura-2017-hacl2] Phytosphingosine degradation pathway includes fatty acid α-oxidation reactions in the endoplasmic reticulum (2017). https://pubmed.ncbi.nlm.nih.gov/28289220/ DOI: 10.1073/pnas.1700138114","model_system":"Human HACL2 immunofluorescence and fractionation.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [kitamura-2017-hacl2] Phytosphingosine degradation pathway includes fatty acid α-oxidation reactions in the endoplasmic reticulum (2017). https://pubmed.ncbi.nlm.nih.gov/28289220/ DOI: 10.1073/pnas.1700138114","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}