{"id":"88e75018-3e1d-55f1-b099-854ef8e6c9d0","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-buffer","predicate":"converts_to","statement":"Muscle Crat deletion reduced short-chain acyltransferase activity and acetylcarnitine pools, supporting transfer of acetyl groups from acetyl-CoA to carnitine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"11bed34f-f4ab-570c-b3fc-31bf39d08c6e","mechanism_event_label":"Carnitine can carry excess acetyl groups as well as long fatty-acid groups.","subject":{"id":"224eacc1-fea4-5737-b661-56e7e41a64e7","slug":"mouse-crat","display_name":"Mouse carnitine acetyltransferase / Crat","entity_type_key":"protein"},"object":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"11bed34f-f4ab-570c-b3fc-31bf39d08c6e","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-buffer-event","event_type":"observed_relationship","label":"Carnitine can carry excess acetyl groups as well as long fatty-acid groups.","description":"Muscle Crat deletion reduced short-chain acyltransferase activity and acetylcarnitine pools, supporting transfer of acetyl groups from acetyl-CoA to carnitine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"224eacc1-fea4-5737-b661-56e7e41a64e7","slug":"mouse-crat","display_name":"Mouse carnitine acetyltransferase / Crat","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and full-text Figures 1-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Muscle-specific mouse knockout, enzyme assays and metabolomics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Crat buffer function depends on tissue and substrate conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Carnitine can carry excess acetyl groups as well as long fatty-acid groups.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Muscle-specific deletion of carnitine acetyltransferase compromises glucose tolerance and metabolic flexibility. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560225/ · DOI 10.1016/j.cmet.2012.04.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"422e29bc-817d-5a50-8147-770c205eba19","evidence_kind":"source_excerpt","locator":"Lines 154-160","start_line":154,"end_line":160,"excerpt":"## l-carnitine-crat-buffer\nCarnitine can carry excess acetyl groups as well as long fatty-acid groups.\nMuscle Crat deletion reduced short-chain acyltransferase activity and acetylcarnitine pools, supporting transfer of acetyl groups from acetyl-CoA to carnitine.\nModel: Muscle-specific mouse knockout, enzyme assays and metabolomics.\nLimitations: Crat buffer function depends on tissue and substrate conditions.\nEvidence access: Primary abstract and full-text Figures 1-4\nMuscle-specific deletion of carnitine acetyltransferase compromises glucose tolerance and metabolic flexibility. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560225/ · DOI 10.1016/j.cmet.2012.04.005","model_system":"Muscle-specific mouse knockout, enzyme assays and metabolomics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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