{"id":"88cf45ee-b002-5085-aa9c-83247908f099","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rela","predicate":"reduces_tested_nuclear_translocation_of","statement":"Cucurbitacin B reduced TNF-alpha-induced RelA nuclear translocation and I-kappa-B-alpha phosphorylation in human A549 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ed0176f0-f8c0-5393-87b9-c7bd30bae06d","mechanism_event_label":"Less inflammatory transcription signaling reached the nucleus.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"fed11f0e-a497-5f77-8d68-7756e1c4b112","slug":"human-rela","display_name":"Human NF-kappa-B subunit RelA / RELA","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ed0176f0-f8c0-5393-87b9-c7bd30bae06d","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rela-event","event_type":"observed_relationship","label":"Less inflammatory transcription signaling reached the nucleus.","description":"Cucurbitacin B reduced TNF-alpha-induced RelA nuclear translocation and I-kappa-B-alpha phosphorylation in human A549 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fed11f0e-a497-5f77-8d68-7756e1c4b112","slug":"human-rela","display_name":"Human NF-kappa-B subunit RelA / RELA","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4b7e6069-53d4-58cf-be3c-d63e362025bf","slug":"nfkbia","display_name":"Human I-kappa-B alpha / NFKBIA","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human A549 cytokine-stimulation assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Downstream readouts do not establish direct inhibition of each protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Less inflammatory transcription signaling reached the nucleus.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B Down-Regulates TNF Receptor 1 Expression and Inhibits the TNF-α-Dependent Nuclear Factor κB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35806134/ · DOI 10.3390/ijms23137130","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1ee01cbe-750d-5cb5-a3eb-dc7f047bbc0f","evidence_kind":"source_excerpt","locator":"Lines 204-210","start_line":204,"end_line":210,"excerpt":"## cucurbitacin-b-rela\nLess inflammatory transcription signaling reached the nucleus.\nCucurbitacin B reduced TNF-alpha-induced RelA nuclear translocation and I-kappa-B-alpha phosphorylation in human A549 cells.\nModel: Human A549 cytokine-stimulation assays.\nLimitations: Downstream readouts do not establish direct inhibition of each protein.\nEvidence access: Primary abstract\nCucurbitacin B Down-Regulates TNF Receptor 1 Expression and Inhibits the TNF-α-Dependent Nuclear Factor κB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35806134/ · DOI 10.3390/ijms23137130","model_system":"Human A549 cytokine-stimulation assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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