{"id":"88976a9e-2b0b-51e2-9224-d049ec8569f5","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-protein-clearance","predicate":"accelerates","statement":"Lithium accelerated clearance of expressed mutant huntingtin and alpha-synuclein in inducible PC12 models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cf1b0550-6c91-52e3-9494-30c11622877c","mechanism_event_label":"Recycling changed the persistence of aggregation-prone proteins.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"c0048233-200a-50e4-acfa-7c93e4547234","slug":"rat-pc12-mutant-protein-clearance","display_name":"Rat PC12-cell clearance of expressed mutant proteins","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cf1b0550-6c91-52e3-9494-30c11622877c","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-protein-clearance-event","event_type":"observed_relationship","label":"Recycling changed the persistence of aggregation-prone proteins.","description":"Lithium accelerated clearance of expressed mutant huntingtin and alpha-synuclein in inducible PC12 models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c0048233-200a-50e4-acfa-7c93e4547234","slug":"rat-pc12-mutant-protein-clearance","display_name":"Rat PC12-cell clearance of expressed mutant proteins","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b01363cb-357a-51f7-8ba9-e5ca23690382","slug":"lithium-chloride","display_name":"Lithium chloride","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat PC12; 10 mM LiCl; expression switched off before clearance measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered protein models do not establish treatment of Huntington or Parkinson disease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Recycling changed the persistence of aggregation-prone proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lithium induces autophagy by inhibiting inositol monophosphatase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16186256/ · DOI 10.1083/jcb.200504035","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"931ea5b2-1f8c-555e-a452-c8733d0271bd","evidence_kind":"source_excerpt","locator":"Lines 96-102","start_line":96,"end_line":102,"excerpt":"## lithium-protein-clearance\nRecycling changed the persistence of aggregation-prone proteins.\nLithium accelerated clearance of expressed mutant huntingtin and alpha-synuclein in inducible PC12 models.\nModel: Rat PC12; 10 mM LiCl; expression switched off before clearance measurement.\nLimitations: Engineered protein models do not establish treatment of Huntington or Parkinson disease.\nEvidence access: Primary full text\nLithium induces autophagy by inhibiting inositol monophosphatase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16186256/ · DOI 10.1083/jcb.200504035","model_system":"Rat PC12; 10 mM LiCl; expression switched off before clearance measurement.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}