{"id":"885d4f28-c89d-5cd7-b79d-0341312a97d2","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-muscle-peroxide","predicate":"attenuates","statement":"Silicon preparations at 0.1–2 mM attenuated peroxide-associated loss of viability and differentiation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"48a3b505-40d5-50bc-b759-f8a80fc3582a","mechanism_event_label":"Oxidative challenge changed the context in which protection was observed.","subject":{"id":"36f2ccf3-5023-5074-aab3-d65d3650b9f6","slug":"metasilicate-derived-culture-solution","display_name":"Sodium-metasilicate-derived soluble silicon culture preparation","entity_type_key":"chemical_species"},"object":{"id":"66bef186-eebb-500b-b833-44577d41901c","slug":"mouse-c2c12-peroxide-injury","display_name":"Peroxide injury in mouse C2C12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"48a3b505-40d5-50bc-b759-f8a80fc3582a","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-muscle-peroxide-event","event_type":"observed_relationship","label":"Oxidative challenge changed the context in which protection was observed.","description":"Silicon preparations at 0.1–2 mM attenuated peroxide-associated loss of viability and differentiation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36f2ccf3-5023-5074-aab3-d65d3650b9f6","slug":"metasilicate-derived-culture-solution","display_name":"Sodium-metasilicate-derived soluble silicon culture preparation","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"66bef186-eebb-500b-b833-44577d41901c","slug":"mouse-c2c12-peroxide-injury","display_name":"Peroxide injury in mouse C2C12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse muscle-cell culture, 24-hour challenge experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not identify a direct antioxidant reaction or an in-vivo effective dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Oxidative challenge changed the context in which protection was observed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ionic Silicon Protects Oxidative Damage and Promotes Skeletal Muscle Cell Regeneration. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33419056/ · DOI 10.3390/ijms22020497","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d520e64a-635c-53e9-84ba-5b08c6d34b5a","evidence_kind":"source_excerpt","locator":"Lines 328-334","start_line":328,"end_line":334,"excerpt":"## silica-muscle-peroxide\nOxidative challenge changed the context in which protection was observed.\nSilicon preparations at 0.1–2 mM attenuated peroxide-associated loss of viability and differentiation.\nModel: Mouse muscle-cell culture, 24-hour challenge experiments.\nLimitations: Does not identify a direct antioxidant reaction or an in-vivo effective dose.\nEvidence access: Primary full text\nIonic Silicon Protects Oxidative Damage and Promotes Skeletal Muscle Cell Regeneration. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33419056/ · DOI 10.3390/ijms22020497","model_system":"Mouse muscle-cell culture, 24-hour challenge experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}