{"id":"88420203-0581-5550-a381-06ec42fb2429","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-gtpbp3-translation","predicate":"knockout_reduces","statement":"Deleting GTPBP3 in human cells eliminated the normal taurine-containing wobble modification and reduced mitochondrial translation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3a60aedd-64eb-5db3-8802-277e457871f1","mechanism_event_label":"Removing the modifying machinery impaired mitochondrial protein production.","subject":{"id":"cd2a6b5d-a6cf-5829-b0e4-b52a0f92d153","slug":"gtpbp3","display_name":"Human mitochondrial GTP-binding protein 3 / GTPBP3","entity_type_key":"protein"},"object":{"id":"491dc607-d474-5f62-95e7-e2a7406e290f","slug":"human-gtpbp3-mitochondrial-translation","display_name":"Mitochondrial translation in human GTPBP3-deficient cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3a60aedd-64eb-5db3-8802-277e457871f1","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-gtpbp3-translation-event","event_type":"observed_relationship","label":"Removing the modifying machinery impaired mitochondrial protein production.","description":"Deleting GTPBP3 in human cells eliminated the normal taurine-containing wobble modification and reduced mitochondrial translation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cd2a6b5d-a6cf-5829-b0e4-b52a0f92d153","slug":"gtpbp3","display_name":"Human mitochondrial GTP-binding protein 3 / GTPBP3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"491dc607-d474-5f62-95e7-e2a7406e290f","slug":"human-gtpbp3-mitochondrial-translation","display_name":"Mitochondrial translation in human GTPBP3-deficient cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dd968147-5f93-56d5-bc83-7fa01f9b0e42","slug":"mt-trna-taurinomethyluridine","display_name":"Mitochondrial tRNA 5-taurinomethyluridine / tau-m5U34","entity_type_key":"rna_modification"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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primary-abstract references and experimental limitations individually identified. 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