{"id":"88259b13-fb93-58b0-a9ff-fdc93fa643eb","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-shigella-membrane","predicate":"disrupts","statement":"At antibacterial concentrations, eugenol depolarized and damaged Shigella flexneri membranes, increased oxidative damage and depleted bacterial ATP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2f8de5cb-fc23-513e-a67d-ce0ace62f332","mechanism_event_label":"At antibacterial concentrations, eugenol depolarized and damaged Shigella flexneri membranes, increased oxidative damage and depleted bacterial ATP.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"2a7d70e1-a8a9-540c-88dd-4bc63a48753f","slug":"shigella-flexneri-membrane-and-atp","display_name":"Shigella flexneri membrane integrity and intracellular ATP","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2f8de5cb-fc23-513e-a67d-ce0ace62f332","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-shigella-membrane-event","event_type":"observed_relationship","label":"At antibacterial concentrations, eugenol depolarized and damaged Shigella flexneri membranes, increased oxidative damage and depleted bacterial ATP.","description":"At antibacterial concentrations, eugenol depolarized and damaged Shigella flexneri membranes, increased oxidative damage and depleted bacterial ATP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2a7d70e1-a8a9-540c-88dd-4bc63a48753f","slug":"shigella-flexneri-membrane-and-atp","display_name":"Shigella flexneri membrane integrity and intracellular ATP","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c7dcaf56-f53c-5987-acf7-1dbab10df539","slug":"shigella-flexneri","display_name":"Shigella flexneri","entity_type_key":"organism"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"MIC 0.5 mg/mL; MBC 0.8 mg/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Growth-curve interval","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Shigella flexneri ATCC 12022 in broth and food matrices","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Food-preservation concentrations and bacterial effects are not human systemic pharmacology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Shigella flexneri ATCC 12022 in broth and food matrices","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"At antibacterial concentrations, eugenol depolarized and damaged Shigella flexneri membranes, increased oxidative damage and depleted bacterial ATP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antibacterial Effect of Eugenol on Shigella flexneri and Its Mechanism. (2022). https://pubmed.ncbi.nlm.nih.gov/36076751/ DOI: 10.3390/foods11172565","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro and food-matrix exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Growth, membrane integrity, membrane potential and ATP","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"83624e9d-9048-5f61-9fa5-35ddce74af51","evidence_kind":"source_excerpt","locator":"Lines 132-141","start_line":132,"end_line":141,"excerpt":"## eugenol-shigella-membrane\nAt antibacterial concentrations, eugenol depolarized and damaged Shigella flexneri membranes, increased oxidative damage and depleted bacterial ATP.\nModel/species: Shigella flexneri ATCC 12022 in broth and food matrices\nTissue/system: Growth, membrane integrity, membrane potential and ATP\nExposure: MIC 0.5 mg/mL; MBC 0.8 mg/mL\nRoute: In vitro and food-matrix exposure\nDuration: Growth-curve interval\nLimits: Food-preservation concentrations and bacterial effects are not human systemic pharmacology.\nPrimary reference: Antibacterial Effect of Eugenol on Shigella flexneri and Its Mechanism. (2022). https://pubmed.ncbi.nlm.nih.gov/36076751/ DOI: 10.3390/foods11172565\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Shigella flexneri ATCC 12022 in broth and food matrices","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}