{"id":"87c5355e-91d7-5677-b38d-b88f21e613e0","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-neuronal-zinc-rescue","predicate":"normalizes_in_tested_model","statement":"NAC restored neuronal glutathione and normalized basal zinc levels in EAAC1-null mice; NAC or zinc chelation reduced ischemia-associated zinc movement, superoxide and neuronal death.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d654fa85-0695-55df-b73b-6c1f9726e04b","mechanism_event_label":"Restoring precursor supply and binding excess labile zinc were distinct rescue routes.","subject":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"6f974c35-34c1-52e6-b008-8d908c88649d","slug":"mouse-hippocampal-labile-zinc","display_name":"Labile zinc in mouse hippocampal neurons","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d654fa85-0695-55df-b73b-6c1f9726e04b","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-neuronal-zinc-rescue-event","event_type":"observed_relationship","label":"Restoring precursor supply and binding excess labile zinc were distinct rescue routes.","description":"NAC restored neuronal glutathione and normalized basal zinc levels in EAAC1-null mice; NAC or zinc chelation reduced ischemia-associated zinc movement, superoxide and neuronal death.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6f974c35-34c1-52e6-b008-8d908c88649d","slug":"mouse-hippocampal-labile-zinc","display_name":"Labile zinc in mouse hippocampal neurons","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f5b92207-a3ca-5e80-8240-bd00c6036474","slug":"mouse-slc1a1","display_name":"Mouse EAAT3 / Slc1a1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse EAAC1-null ischemia model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"NAC and chelators are separate interventions; the study does not show that extra dietary cysteine and zinc are synergistic.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Restoring precursor supply and binding excess labile zinc were distinct rescue routes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"EAAC1 gene deletion alters zinc homeostasis and exacerbates neuronal injury after transient cerebral ischemia. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21084597/ · DOI 10.1523/JNEUROSCI.2084-10.2010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"9116132a-4ff1-54ff-9261-949fe7300de8","evidence_kind":"source_excerpt","locator":"Lines 124-130","start_line":124,"end_line":130,"excerpt":"## l-cysteine-neuronal-zinc-rescue\nRestoring precursor supply and binding excess labile zinc were distinct rescue routes.\nNAC restored neuronal glutathione and normalized basal zinc levels in EAAC1-null mice; NAC or zinc chelation reduced ischemia-associated zinc movement, superoxide and neuronal death.\nModel: Mouse EAAC1-null ischemia model.\nLimitations: NAC and chelators are separate interventions; the study does not show that extra dietary cysteine and zinc are synergistic.\nEvidence access: Primary abstract\nEAAC1 gene deletion alters zinc homeostasis and exacerbates neuronal injury after transient cerebral ischemia. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21084597/ · DOI 10.1523/JNEUROSCI.2084-10.2010","model_system":"Mouse EAAC1-null ischemia model.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f722669e54eab08ffe7289f9d79ddfc443014ed8c4bc5ba10ce2635470d22498","revision_id":"8ae25609-a031-5782-9c62-6cad8767ea46","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}