{"id":"87bd11a3-ced9-543a-9f27-02527fe2a7b8","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-tet1-knockdown","predicate":"knockdown_reduces","statement":"TET1 knockdown reduced luteolin-associated sub-G1 accumulation and nuclear fragmentation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cbd29a29-8f25-513b-8140-b72ff482aabd","mechanism_event_label":"Removing machinery weakened the observed cell-death response.","subject":{"id":"5fcc7eb2-480f-576e-9028-cb5f8f8a6d73","slug":"tet1","display_name":"TET1","entity_type_key":"protein"},"object":{"id":"1703023b-bf3c-55be-a6e7-5064bd191d5c","slug":"human-colon-luteolin-apoptosis","display_name":"Human colon-cell luteolin-associated apoptosis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cbd29a29-8f25-513b-8140-b72ff482aabd","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-tet1-knockdown-event","event_type":"observed_relationship","label":"Removing machinery weakened the observed cell-death response.","description":"TET1 knockdown reduced luteolin-associated sub-G1 accumulation and nuclear fragmentation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5fcc7eb2-480f-576e-9028-cb5f8f8a6d73","slug":"tet1","display_name":"TET1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1703023b-bf3c-55be-a6e7-5064bd191d5c","slug":"human-colon-luteolin-apoptosis","display_name":"Human colon-cell luteolin-associated apoptosis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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unverified.","entity":null}],"evidence":[{"id":"4b5d137f-4ea6-5ffb-8841-7a03f8f49f29","evidence_kind":"source_excerpt","locator":"Lines 492-498","start_line":492,"end_line":498,"excerpt":"## luteolin-tet1-knockdown\nRemoving machinery weakened the observed cell-death response.\nTET1 knockdown reduced luteolin-associated sub-G1 accumulation and nuclear fragmentation.\nModel: Human colon cancer siRNA experiment.\nLimitations: No clinical efficacy or nutrient-supplement rescue established.\nEvidence access: Primary full text\nLuteolin promotes apoptotic cell death via upregulation of Nrf2 expression by DNA demethylase and the interaction of Nrf2 with p53 in human colon cancer cells. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30988303/ · DOI 10.1038/s12276-019-0238-y","model_system":"Human colon cancer siRNA experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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