{"id":"876b50cf-64ce-59dd-bdb9-bf2dd63d93fb","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:rankl-promotes-osteoclast-differentiation","predicate":"promotes","statement":"Soluble RANKL/ODF supported osteoclast-like-cell formation from mouse spleen precursors in the presence of M-CSF without osteoblast/stromal coculture.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d4fb3f9b-fba3-5b33-83b7-2bf134f1510a","mechanism_event_label":"A bone-cell signal can instruct precursors to become bone-resorbing cells.","subject":{"id":"de7ad036-388f-596d-8766-72afe51b4a67","slug":"tnfsf11","display_name":"RANK ligand / TNFSF11","entity_type_key":"protein"},"object":{"id":"a4c1aaa0-3366-5bb2-bc5c-910241d8b64b","slug":"osteoclast-differentiation","display_name":"Osteoclast differentiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d4fb3f9b-fba3-5b33-83b7-2bf134f1510a","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:rankl-promotes-osteoclast-differentiation-event","event_type":"biochemical_relationship","label":"A bone-cell signal can instruct precursors to become bone-resorbing cells.","description":"Soluble RANKL/ODF supported osteoclast-like-cell formation from mouse spleen precursors in the presence of M-CSF without osteoblast/stromal coculture.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"de7ad036-388f-596d-8766-72afe51b4a67","slug":"tnfsf11","display_name":"RANK ligand / TNFSF11","entity_type_key":"protein"},"role":"differentiation_ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dd170554-eb88-57e5-a77a-87eac770007e","slug":"csf1","display_name":"Macrophage colony-stimulating factor / CSF1","entity_type_key":"protein"},"role":"supporting_growth_factor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a4c1aaa0-3366-5bb2-bc5c-910241d8b64b","slug":"osteoclast-differentiation","display_name":"Osteoclast differentiation","entity_type_key":"cellular_process"},"role":"outcome","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"404fc748-a4da-5661-b3dd-690db50d5a09","slug":"osteoclast","display_name":"Osteoclast","entity_type_key":"cell_type"},"role":"differentiated_cell","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant ligand culture assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"M-CSF and culture context are required; this is not a calcium-supplement response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A bone-cell signal can instruct precursors to become bone-resorbing cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[yasuda1998] Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC19881/ DOI: 10.1073/pnas.95.7.3597","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Spleen-derived osteoclast precursors","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fde14d5f-4adc-5846-aef1-a7f432e88d13","evidence_kind":"source_excerpt","locator":"Lines 1036-1045","start_line":1036,"end_line":1045,"excerpt":"### rankl-promotes-osteoclast-differentiation\nSoluble RANKL/ODF supported osteoclast-like-cell formation from mouse spleen precursors in the presence of M-CSF without osteoblast/stromal coculture.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A bone-cell signal can instruct precursors to become bone-resorbing cells.\norganism: Mus musculus\ntissue_or_cell_type: Spleen-derived osteoclast precursors\nexperimental_model: Recombinant ligand culture assay\nlimitations: M-CSF and culture context are required; this is not a calcium-supplement response.\n[yasuda1998] Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC19881/ DOI: 10.1073/pnas.95.7.3597","model_system":"Recombinant ligand culture assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [yasuda1998] Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL (1998). https://pmc.ncbi.nlm.nih.gov/articles/PMC19881/ DOI: 10.1073/pnas.95.7.3597","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}