{"id":"873245c6-f759-579f-ac7c-40099034b18e","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-ascorbate-repair","predicate":"reduces","statement":"In pulse-radiolysis assays, ascorbate rapidly reduced the one-electron-oxidized ergothioneine transient back toward ergothioneine, forming ascorbyl radicals.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"20a57b9d-056e-538f-bb1f-3088a3a8e0c6","mechanism_event_label":"Vitamin C can repair an oxidized ergothioneine intermediate.","subject":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"object":{"id":"8eb71d06-4815-5a2e-a110-0d6fa2e073a0","slug":"ergothioneine-one-electron-oxidation-product","display_name":"Ergothioneine one-electron oxidation transient","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"20a57b9d-056e-538f-bb1f-3088a3a8e0c6","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-ascorbate-repair-event","event_type":"observed_relationship","label":"Vitamin C can repair an oxidized ergothioneine intermediate.","description":"In pulse-radiolysis assays, ascorbate rapidly reduced the one-electron-oxidized ergothioneine transient back toward ergothioneine, forming ascorbyl radicals.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8eb71d06-4815-5a2e-a110-0d6fa2e073a0","slug":"ergothioneine-one-electron-oxidation-product","display_name":"Ergothioneine one-electron oxidation transient","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"17a4b1a5-451a-563f-8f20-2de8f4b0af8e","slug":"ascorbyl-radical","display_name":"Ascorbyl radical","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free reaction; reported rate constant 6.3 x 10^8 M^-1 s^-1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chemical kinetics do not establish tissue flux or a required supplement combination.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Vitamin C can repair an oxidized ergothioneine intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"One-electron oxidation of ergothioneine and analogues investigated by pulse radiolysis: redox reaction involving ergothioneine and vitamin C. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8615839/ · DOI 10.1042/bj3150625","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"af927fcc-9059-59a6-ae95-3c4d9a5e732b","evidence_kind":"source_excerpt","locator":"Lines 112-118","start_line":112,"end_line":118,"excerpt":"## ergothioneine-ascorbate-repair\nVitamin C can repair an oxidized ergothioneine intermediate.\nIn pulse-radiolysis assays, ascorbate rapidly reduced the one-electron-oxidized ergothioneine transient back toward ergothioneine, forming ascorbyl radicals.\nModel: Cell-free reaction; reported rate constant 6.3 x 10^8 M^-1 s^-1.\nLimitations: Chemical kinetics do not establish tissue flux or a required supplement combination.\nEvidence access: Primary abstract\nOne-electron oxidation of ergothioneine and analogues investigated by pulse radiolysis: redox reaction involving ergothioneine and vitamin C. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8615839/ · DOI 10.1042/bj3150625","model_system":"Cell-free reaction; reported rate constant 6.3 x 10^8 M^-1 s^-1.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}