{"id":"87292208-8b92-52c1-88e4-9bed6a3caec1","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-pparg-expression-t10","predicate":"reduces_expression","statement":"Adding t10,c12 CLA lowered PPAR-gamma and several target-gene transcripts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8aaa27ec-cadb-525a-bb71-4b3699efd647","mechanism_event_label":"A regulator of fat-cell identity was suppressed.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8aaa27ec-cadb-525a-bb71-4b3699efd647","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-pparg-expression-t10-event","event_type":"observed_relationship","label":"A regulator of fat-cell identity was suppressed.","description":"Adding t10,c12 CLA lowered PPAR-gamma and several target-gene transcripts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary cultures of differentiating human preadipocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression and direct ligand binding are different measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A regulator of fat-cell identity was suppressed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Isomer-specific regulation of metabolism and PPARgamma signaling by CLA in human preadipocytes. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12730300/ · DOI 10.1194/jlr.M300001-JLR200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b1453ddc-bc6a-55ce-9061-839bc0d6f5e4","evidence_kind":"source_excerpt","locator":"Lines 86-92","start_line":86,"end_line":92,"excerpt":"## cla-pparg-expression-t10\nA regulator of fat-cell identity was suppressed.\nAdding t10,c12 CLA lowered PPAR-gamma and several target-gene transcripts.\nModel: Primary cultures of differentiating human preadipocytes.\nLimitations: Expression and direct ligand binding are different measurements.\nEvidence access: Primary abstract\nIsomer-specific regulation of metabolism and PPARgamma signaling by CLA in human preadipocytes. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12730300/ · DOI 10.1194/jlr.M300001-JLR200","model_system":"Primary cultures of differentiating human preadipocytes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}