{"id":"8708958a-eedd-55b6-af83-77495f5b15f6","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-high-medium-nlrp3-block","predicate":"inhibits","statement":"Raising extracellular K to 45 mM maximally inhibited NLRP3 responses to the tested toxins and particles.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7b58bee0-c169-56c1-8601-bea653299c07","mechanism_event_label":"Reducing the outward potassium gradient blocked these activation routes.","subject":{"id":"65e7fcad-670f-5dba-8488-896c86f2bbae","slug":"extracellular-potassium-concentration","display_name":"Extracellular potassium concentration","entity_type_key":"cellular_process"},"object":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"7b58bee0-c169-56c1-8601-bea653299c07","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-high-medium-nlrp3-block-event","event_type":"biochemical_relationship","label":"Reducing the outward potassium gradient blocked these activation routes.","description":"Raising extracellular K to 45 mM maximally inhibited NLRP3 responses to the tested toxins and particles.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"gradient-setting ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b53e832d-0776-5495-b5cd-cb6a8e8c538b","slug":"nlrp3","display_name":"NLRP3","entity_type_key":"protein"},"role":"sensor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eb449605-97b9-531c-afeb-5c3ac08897a8","slug":"nigericin","display_name":"Nigericin","entity_type_key":"small_molecule"},"role":"one tested trigger","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"separate extracellular trigger","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"65e7fcad-670f-5dba-8488-896c86f2bbae","slug":"extracellular-potassium-concentration","display_name":"Extracellular potassium concentration","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Primed mouse macrophages, extracellular-K titration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Artificial extracellular potassium elevation; 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topical membership is not evidence of a direct dietary effect.\nplain_language: Reducing the outward potassium gradient blocked these activation routes.\norganism: Mouse\ntissue_or_cell_type: Bone-marrow macrophages\nexperimental_model: Primed mouse macrophages, extracellular-K titration.\nlimitations: Stimulus-specific; does not justify elevating blood potassium.\nexposure: Artificial extracellular potassium elevation; not dietary excess.\n[munoz-2013-nlrp3] K+ efflux is the Common Trigger of NLRP3 inflammasome Activation by Bacterial Toxins and Particulate Matter (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3730833/ DOI: 10.1016/j.immuni.2013.05.016","model_system":"Primed mouse macrophages, extracellular-K titration.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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