{"id":"86da3b34-829f-5e5c-ad1d-4378d224d9c1","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-cndp2-lacphe","predicate":"knockout_reduces","statement":"Deleting CNDP2 in human RT4 bladder epithelial cells reduced Lac-Phe production; extracellular lactate stimulated production in the control cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"10c08967-f370-527d-bb46-016f07dda5e7","mechanism_event_label":"The peptide-processing enzyme also participates in another metabolic pathway.","subject":{"id":"66621280-d7e5-5e8b-8ae1-873711628d87","slug":"human-cndp2","display_name":"Human cytosolic nonspecific dipeptidase / CNDP2","entity_type_key":"protein"},"object":{"id":"2e1e9f48-df5d-5d8a-a1e9-96e90a6eb6d1","slug":"n-lactoyl-phenylalanine","display_name":"N-Lactoyl-phenylalanine / Lac-Phe","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"10c08967-f370-527d-bb46-016f07dda5e7","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-cndp2-lacphe-event","event_type":"observed_relationship","label":"The peptide-processing enzyme also participates in another metabolic pathway.","description":"Deleting CNDP2 in human RT4 bladder epithelial cells reduced Lac-Phe production; extracellular lactate stimulated production in the control cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"66621280-d7e5-5e8b-8ae1-873711628d87","slug":"human-cndp2","display_name":"Human cytosolic nonspecific dipeptidase / CNDP2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2e1e9f48-df5d-5d8a-a1e9-96e90a6eb6d1","slug":"n-lactoyl-phenylalanine","display_name":"N-Lactoyl-phenylalanine / Lac-Phe","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and PMC full-text Figure 2 and CRISPR methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human RT4 cell knockout and 25 mM lactate treatment for 24 hours; Figure 2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a shared-enzyme connection, not proof that carnosine changes appetite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The peptide-processing enzyme also participates in another metabolic pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"An exercise-inducible metabolite that suppresses feeding and obesity. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35705806/ · DOI 10.1038/s41586-022-04828-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"372dd245-8ca5-557f-ae88-42112f81e14d","evidence_kind":"source_excerpt","locator":"Lines 172-178","start_line":172,"end_line":178,"excerpt":"## carnosine-cndp2-lacphe\nThe peptide-processing enzyme also participates in another metabolic pathway.\nDeleting CNDP2 in human RT4 bladder epithelial cells reduced Lac-Phe production; extracellular lactate stimulated production in the control cells.\nModel: Human RT4 cell knockout and 25 mM lactate treatment for 24 hours; Figure 2.\nLimitations: This is a shared-enzyme connection, not proof that carnosine changes appetite.\nEvidence access: Primary abstract and PMC full-text Figure 2 and CRISPR methods\nAn exercise-inducible metabolite that suppresses feeding and obesity. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35705806/ · DOI 10.1038/s41586-022-04828-5","model_system":"Human RT4 cell knockout and 25 mM lactate treatment for 24 hours; Figure 2.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"469cbc38203db56bd031f9d8a2dd2bfa387713950ca30199511cd137e927aa7a","revision_id":"cc409a15-8b04-50ee-a7ff-d1bf32f686bb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}