{"id":"86c4b39a-1840-5ce8-b8dc-12e8bb6010b1","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:epsp-production","predicate":"converted_to_in_recorded_reaction","statement":"EPSPS uses PEP and shikimate-3-phosphate to produce EPSP and phosphate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"4c3d9467-8f31-5389-974a-5ae601e97b59","mechanism_event_label":"EPSPS uses PEP and shikimate-3-phosphate to produce EPSP and phosphate.","subject":{"id":"ba13ffaa-56b5-5976-945c-71c0921defd8","slug":"shikimate-3-phosphate","display_name":"Shikimate 3-phosphate","entity_type_key":"small_molecule"},"object":{"id":"e27b2697-e6fe-59a6-ace0-69924b289e20","slug":"epsp","display_name":"5-Enolpyruvylshikimate 3-phosphate / EPSP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"4c3d9467-8f31-5389-974a-5ae601e97b59","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:epsp-production-event","event_type":"biochemical_reaction","label":"EPSPS uses PEP and shikimate-3-phosphate to produce EPSP and phosphate.","description":"**EPSPS uses a second PEP molecule.** E. coli EPSPS (AroA) transfers an enolpyruvyl group from a second PEP molecule to shikimate-3-phosphate, making 5-enolpyruvylshikimate-3-phosphate (EPSP) and phosphate. Glyphosate inhibits this enzyme by occupying the PEP-associated substrate site in the substrate-bound enzyme. Purified-enzyme IC50 estimates were about 2.5 µM for E. coli EPSPS and 11 mM for CP4 EPSPS. These are not interchangeable microbial-growth thresholds or human intestinal effect concentrations. Adding shikimate upstream does not remove the need for EPSPS downstream. [Molecular basis for the herbicide resistance of Roundup Ready crops.](https://pubmed.ncbi.nlm.nih.gov/16916934/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ba13ffaa-56b5-5976-945c-71c0921defd8","slug":"shikimate-3-phosphate","display_name":"Shikimate 3-phosphate","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e27b2697-e6fe-59a6-ace0-69924b289e20","slug":"epsp","display_name":"5-Enolpyruvylshikimate 3-phosphate / EPSP","entity_type_key":"small_molecule"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5b6fca80-44de-5da7-985f-8783f9302c24","slug":"ecoli-aroa","display_name":"Escherichia coli EPSP synthase / AroA","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"20e5852c-38a1-54be-b486-d3d8083d9c2e","slug":"phosphoenolpyruvate","display_name":"Phosphoenolpyruvate / PEP","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text HTML and indexed abstract reviewed for the stated results; archived locally.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Escherichia coli biochemical pathway; source-specific enzyme evidence recorded in the passage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. 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