{"id":"86349a5e-cb09-5aa8-b1e1-e093c5c9accb","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-complex-i-q","predicate":"reduces","statement":"Complex I transferred electrons from NADH to ubiquinone-10 in reconstituted membranes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"852af1be-5ce2-5501-86cc-b920fcc7bbca","mechanism_event_label":"CoQ receives electrons from the first respiratory complex.","subject":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"object":{"id":"64f0151b-6c0d-5adf-a6b4-13056bc8dfb3","slug":"ubiquinone-10","display_name":"Ubiquinone-10","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"852af1be-5ce2-5501-86cc-b920fcc7bbca","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-complex-i-q-event","event_type":"biochemical_relationship","label":"CoQ receives electrons from the first respiratory complex.","description":"Complex I transferred electrons from NADH to ubiquinone-10 in reconstituted membranes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"electron_donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"64fbe9b3-bf24-5b66-9611-8b78262d6ffc","slug":"reduced-coq10","display_name":"Reduced CoQ10","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"64f0151b-6c0d-5adf-a6b4-13056bc8dfb3","slug":"ubiquinone-10","display_name":"Ubiquinone-10","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/29133414.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"139122e8a567596e6ec1b4bd361567d0e5cd4e3329fec72d47b949a86d29610d\", \"start_char\": 0, \"end_char\": 1770, \"text_sha256\": \"139122e8a567596e6ec1b4bd361567d0e5cd4e3329fec72d47b949a86d29610d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Proteoliposome enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Ubiquinones with one to ten isoprenoid units","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified enzyme system; short-chain analogues do not have identical binding and release kinetics to Q10.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mammalian complex I preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CoQ receives electrons from the first respiratory complex.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p29133414] Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I. (2017). https://pubmed.ncbi.nlm.nih.gov/29133414/ DOI: 10.1073/pnas.1714074114","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Membrane quinone channel","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6e12d2ba-6cc0-5faf-8bd3-9445a23ab961","evidence_kind":"source_excerpt","locator":"Lines 385-396","start_line":385,"end_line":396,"excerpt":"### coq10-complex-i-q\nComplex I transferred electrons from NADH to ubiquinone-10 in reconstituted membranes.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CoQ receives electrons from the first respiratory complex.\norganism: Mammalian complex I preparation\ntissue_or_cell_type: Membrane quinone channel\nexperimental_model: Proteoliposome enzyme kinetics\nlimitations: Purified enzyme system; short-chain analogues do not have identical binding and release kinetics to Q10.\nexposure: Ubiquinones with one to ten isoprenoid units\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/29133414.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"139122e8a567596e6ec1b4bd361567d0e5cd4e3329fec72d47b949a86d29610d\", \"start_char\": 0, \"end_char\": 1770, \"text_sha256\": \"139122e8a567596e6ec1b4bd361567d0e5cd4e3329fec72d47b949a86d29610d\"}\n[coq10-p29133414] Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I. 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