{"id":"86107ba6-e7ff-5901-8284-1b87ae0e79d1","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-atp13a2-export","predicate":"mediates","statement":"ATP13A2 supports lysosomal polyamine export to cytosol after endocytic uptake; spermine had the highest tested affinity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3b56b5a1-ad72-5ce5-83f0-e0e581eb97db","mechanism_event_label":"A molecule can enter a cell yet remain trapped in a compartment.","subject":{"id":"409f2bb7-f510-56e1-8f03-a8b9587af3df","slug":"atp13a2","display_name":"Human ATP13A2 / PARK9","entity_type_key":"protein"},"object":{"id":"48bc0bab-aace-567c-96ac-a1ece2793677","slug":"human-lysosomal-polyamine-export","display_name":"Human lysosome-to-cytosol polyamine export","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3b56b5a1-ad72-5ce5-83f0-e0e581eb97db","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-atp13a2-export-event","event_type":"observed_relationship","label":"A molecule can enter a cell yet remain trapped in a compartment.","description":"ATP13A2 supports lysosomal polyamine export to cytosol after endocytic uptake; spermine had the highest tested affinity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"409f2bb7-f510-56e1-8f03-a8b9587af3df","slug":"atp13a2","display_name":"Human ATP13A2 / PARK9","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"48bc0bab-aace-567c-96ac-a1ece2793677","slug":"human-lysosomal-polyamine-export","display_name":"Human lysosome-to-cytosol polyamine export","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ded80d0b-9076-5034-9e87-cd38f7dafae5","slug":"spermine","display_name":"Spermine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human transporter and cellular transport experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Polyamine-class result; strongest affinity was for spermine, not necessarily spermidine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A molecule can enter a cell yet remain trapped in a compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"ATP13A2 deficiency disrupts lysosomal polyamine export. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31996848/ · DOI 10.1038/s41586-020-1968-7","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c6bf2ad6-c724-5a31-9571-5edc27f488a6","evidence_kind":"source_excerpt","locator":"Lines 118-124","start_line":118,"end_line":124,"excerpt":"## spermidine-atp13a2-export\nA molecule can enter a cell yet remain trapped in a compartment.\nATP13A2 supports lysosomal polyamine export to cytosol after endocytic uptake; spermine had the highest tested affinity.\nModel: Purified human transporter and cellular transport experiments.\nLimitations: Polyamine-class result; strongest affinity was for spermine, not necessarily spermidine.\nEvidence access: Primary abstract\nATP13A2 deficiency disrupts lysosomal polyamine export. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31996848/ · DOI 10.1038/s41586-020-1968-7","model_system":"Purified human transporter and cellular transport experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}