{"id":"86105900-15bc-56d1-9170-eda53a11d257","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-keap1-cysteines","predicate":"modifies_cysteines_on","statement":"Revised mass spectrometry detected sulforaphane modification of at least four KEAP1 cysteines, including readily modified C151.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3bc613da-426d-5f4a-9aa9-5ab049315502","mechanism_event_label":"The molecule changes a sensor protein that controls the stress-response program.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"c5b67b38-fe93-5e8f-91af-d77d3f590d98","slug":"keap1","display_name":"Human KEAP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"3bc613da-426d-5f4a-9aa9-5ab049315502","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-keap1-cysteines-event","event_type":"biochemical_relationship","label":"The molecule changes a sensor protein that controls the stress-response program.","description":"Revised mass spectrometry detected sulforaphane modification of at least four KEAP1 cysteines, including readily modified C151.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c5b67b38-fe93-5e8f-91af-d77d3f590d98","slug":"keap1","display_name":"Human KEAP1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/21391649.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac\", \"start_char\": 0, \"end_char\": 1511, \"text_sha256\": \"d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mass spectrometry with revised sample preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane exposure; omission of iodoacetamide and shorter processing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Explains an assay-dependent detection problem; not an unresolved universal C151 disagreement or a human efficacy trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"KEAP1 protein cysteine mapping","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The molecule changes a sensor protein that controls the stress-response program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p21391649] Modification of keap1 cysteine residues by sulforaphane. (2011). https://pubmed.ncbi.nlm.nih.gov/21391649/ DOI: 10.1021/tx100389r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Reversible electrophile-thiol adducts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9efbe431-e1ec-5251-8d1a-6fe8ed632d34","evidence_kind":"source_excerpt","locator":"Lines 424-435","start_line":424,"end_line":435,"excerpt":"### sulforaphane-keap1-cysteines\nRevised mass spectrometry detected sulforaphane modification of at least four KEAP1 cysteines, including readily modified C151.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The molecule changes a sensor protein that controls the stress-response program.\norganism: KEAP1 protein cysteine mapping\ntissue_or_cell_type: Reversible electrophile-thiol adducts\nexperimental_model: Mass spectrometry with revised sample preparation\nlimitations: Explains an assay-dependent detection problem; not an unresolved universal C151 disagreement or a human efficacy trial.\nexposure: Sulforaphane exposure; omission of iodoacetamide and shorter processing\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/21391649.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac\", \"start_char\": 0, \"end_char\": 1511, \"text_sha256\": \"d1ecbe68352504813e0a666dc7474fba7f0a3f3aeb5d0b2fea681d376df999ac\"}\n[sulforaphane-p21391649] Modification of keap1 cysteine residues by sulforaphane. 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