{"id":"85f5329a-283a-5195-af48-7fe1bbcfa903","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-gaba-at-iron-cluster","predicate":"contains","statement":"Native and inhibitor-bound pig liver GABA-AT structures resolved a [2Fe-2S] cluster near the PLP sites.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"78d135f9-cf18-5475-998e-3a079ff9a148","mechanism_event_label":"Iron and B6 occur in the same enzyme structure.","subject":{"id":"4f496840-b2dd-5317-a6f5-72b2409b443b","slug":"pig-abat","display_name":"Pig GABA aminotransferase / ABAT","entity_type_key":"protein"},"object":{"id":"02107865-f169-5624-9ea4-9448a4cce185","slug":"iron-sulfur-2fe2s","display_name":"[2Fe-2S] iron-sulfur cluster","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"78d135f9-cf18-5475-998e-3a079ff9a148","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-gaba-at-iron-cluster-event","event_type":"biochemical_relationship","label":"Iron and B6 occur in the same enzyme structure.","description":"Native and inhibitor-bound pig liver GABA-AT structures resolved a [2Fe-2S] cluster near the PLP sites.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4f496840-b2dd-5317-a6f5-72b2409b443b","slug":"pig-abat","display_name":"Pig GABA aminotransferase / ABAT","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"02107865-f169-5624-9ea4-9448a4cce185","slug":"iron-sulfur-2fe2s","display_name":"[2Fe-2S] iron-sulfur cluster","entity_type_key":"chemical_species"},"role":"structural-cluster","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B6 and iron-sulfur cluster coexistence; functional nutritional interaction remains untested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pig liver GABA aminotransferase; inhibitor complexes and spectroscopy","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Native and inhibitor-bound enzyme crystallography.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The cluster function was unknown; no iron-deficiency or B6-rescue response was demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Sus scrofa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Iron and B6 occur in the same enzyme structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[storici-2004-gaba-at] Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin (2004). https://doi.org/10.1074/jbc.M305884200 DOI: 10.1074/jbc.M305884200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Pig liver enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"584041bc-313a-5460-bcc3-9c4e3b91ae9c","evidence_kind":"source_excerpt","locator":"Lines 1083-1094","start_line":1083,"end_line":1094,"excerpt":"### b6-neuro-gaba-at-iron-cluster\nNative and inhibitor-bound pig liver GABA-AT structures resolved a [2Fe-2S] cluster near the PLP sites.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Iron and B6 occur in the same enzyme structure.\norganism: Sus scrofa\ntissue_or_cell_type: Pig liver enzyme\nexperimental_model: Pig liver GABA aminotransferase; inhibitor complexes and spectroscopy\nlimitations: The cluster function was unknown; no iron-deficiency or B6-rescue response was demonstrated.\nexposure: Native and inhibitor-bound enzyme crystallography.\ncross_nutrient: B6 and iron-sulfur cluster coexistence; functional nutritional interaction remains untested.\n[storici-2004-gaba-at] Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin (2004). https://doi.org/10.1074/jbc.M305884200 DOI: 10.1074/jbc.M305884200","model_system":"Pig liver GABA aminotransferase; inhibitor complexes and spectroscopy","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [storici-2004-gaba-at] Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin (2004). https://doi.org/10.1074/jbc.M305884200 DOI: 10.1074/jbc.M305884200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}