{"id":"85a9e2fa-8172-58ac-a830-b421c75014e1","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-induced-iron-response-failure","predicate":"did_not_restore_reticulocytes_during_induced_b2_deficiency","statement":"In two patients with induced B2 deficiency, parenteral iron did not trigger reticulocytosis, whereas subsequent riboflavin did.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4ae4ddcc-96a0-55e4-ba36-f28685f95424","mechanism_event_label":"More iron did not restore new red-cell production while usable B2 was severely limited in these cases.","subject":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"object":{"id":"376fcaa7-a41d-5649-90aa-fb7deac06416","slug":"erythroid-cell-production","display_name":"Erythroid cell production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4ae4ddcc-96a0-55e4-ba36-f28685f95424","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-induced-iron-response-failure-event","event_type":"observed_intervention","label":"More iron did not restore new red-cell production while usable B2 was severely limited in these cases.","description":"In two patients with induced B2 deficiency, parenteral iron did not trigger reticulocytosis, whereas subsequent riboflavin did.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subsequent_intervention","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"da6825a9-c81a-5f18-bb3d-11401d04eec8","slug":"galactoflavin","display_name":"Galactoflavin","entity_type_key":"small_molecule"},"role":"antagonist","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"376fcaa7-a41d-5649-90aa-fb7deac06416","slug":"erythroid-cell-production","display_name":"Erythroid cell production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Documented failure of iron response in a specific induced B2-deficiency setting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Historical induced-depletion study in six men with inoperable cancers; no healthy randomized control group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Low-riboflavin semisynthetic diet plus galactoflavin antagonist, followed by riboflavin replacement; three also received oral antibiotics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Two cases within a six-person cancer/galactoflavin experiment; cannot generalize this to every anemia or infer a modern treatment regimen.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"More iron did not restore new red-cell production while usable B2 was severely limited in these cases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b2-lane1964] THE RAPID INDUCTION OF HUMAN RIBOFLAVIN DEFICIENCY WITH GALACTOFLAVIN (1964). https://pubmed.ncbi.nlm.nih.gov/14135487/ DOI: 10.1172/jci104921","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Circulating reticulocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fa1a3299-6b29-50d9-a5ab-07fad8eba65b","evidence_kind":"source_excerpt","locator":"Lines 1814-1825","start_line":1814,"end_line":1825,"excerpt":"### b2-induced-iron-response-failure\nIn two patients with induced B2 deficiency, parenteral iron did not trigger reticulocytosis, whereas subsequent riboflavin did.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: More iron did not restore new red-cell production while usable B2 was severely limited in these cases.\norganism: Homo sapiens\ntissue_or_cell_type: Circulating reticulocytes\nexperimental_model: Historical induced-depletion study in six men with inoperable cancers; no healthy randomized control group.\nlimitations: Two cases within a six-person cancer/galactoflavin experiment; cannot generalize this to every anemia or infer a modern treatment regimen.\nexposure: Low-riboflavin semisynthetic diet plus galactoflavin antagonist, followed by riboflavin replacement; three also received oral antibiotics.\ncross_nutrient: Documented failure of iron response in a specific induced B2-deficiency setting.\n[b2-lane1964] THE RAPID INDUCTION OF HUMAN RIBOFLAVIN DEFICIENCY WITH GALACTOFLAVIN (1964). https://pubmed.ncbi.nlm.nih.gov/14135487/ DOI: 10.1172/jci104921","model_system":"Historical induced-depletion study in six men with inoperable cancers; no healthy randomized control group.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b2-lane1964] THE RAPID INDUCTION OF HUMAN RIBOFLAVIN DEFICIENCY WITH GALACTOFLAVIN (1964). https://pubmed.ncbi.nlm.nih.gov/14135487/ DOI: 10.1172/jci104921","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}