{"id":"85458f67-baa9-56fb-8b82-cf80ec84bce1","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-vitc-coalation","predicate":"pretreatment_reduces","statement":"Vitamin C pretreatment reduced diamide-induced anti-CoA protein immunoreactivity by approximately 50% in HEK293/Pank1beta cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c8060194-2550-53d1-9fde-cbb8e82f8013","mechanism_event_label":"Vitamin C exposure blunted the measured CoA protein modification during this experimental oxidative challenge.","subject":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"object":{"id":"9cb713ef-3378-5882-934c-bd037e5edadc","slug":"protein-coalation","display_name":"Protein cysteine CoAlation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c8060194-2550-53d1-9fde-cbb8e82f8013","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-vitc-coalation-event","event_type":"biochemical_relationship","label":"Vitamin C exposure blunted the measured CoA protein modification during this experimental oxidative challenge.","description":"Vitamin C pretreatment reduced diamide-induced anti-CoA protein immunoreactivity by approximately 50% in HEK293/Pank1beta cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"protein_modifier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f27a6fe-065b-5a1f-b015-b778b1ad5831","slug":"diamide","display_name":"Diamide thiol oxidant","entity_type_key":"small_molecule"},"role":"oxidative_challenge","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1f79fec8-f7a0-5537-a03a-e74fb90b6c0b","slug":"protein-cysteine-coa-disulfide","display_name":"Protein cysteine–CoA mixed disulfides","entity_type_key":"protein_state"},"role":"assayed_modification","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9cb713ef-3378-5882-934c-bd037e5edadc","slug":"protein-coalation","display_name":"Protein cysteine CoAlation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 29 and 117; Fig. 2E","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Antioxidant pretreatment of engineered HEK293/Pank1beta cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"1 mM vitamin C for 2 hours before 0.5 mM diamide for 30 minutes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The endpoint is protein anti-CoA immunoreactivity, not clinical benefit; lower CoAlation is not assumed universally beneficial. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin C exposure blunted the measured CoA protein modification during this experimental oxidative challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Engineered HEK293 cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"39314d67-acdd-52a0-b8c9-75de9e09cc6f","evidence_kind":"source_excerpt","locator":"Lines 1075-1087","start_line":1075,"end_line":1087,"excerpt":"### b5-met-vitc-coalation\nVitamin C pretreatment reduced diamide-induced anti-CoA protein immunoreactivity by approximately 50% in HEK293/Pank1beta cells.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin C exposure blunted the measured CoA protein modification during this experimental oxidative challenge.\norganism: Homo sapiens\ntissue_or_cell_type: Engineered HEK293 cultures\nexperimental_model: Antioxidant pretreatment of engineered HEK293/Pank1beta cells\nlimitations: The endpoint is protein anti-CoA immunoreactivity, not clinical benefit; lower CoAlation is not assumed universally beneficial. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: 1 mM vitamin C for 2 hours before 0.5 mM diamide for 30 minutes.\ncross_nutrient: true\nevidence_location: Full text lines 29 and 117; Fig. 2E\n[b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. (2017). https://pubmed.ncbi.nlm.nih.gov/28341808/ DOI: 10.1042/bcj20170129","model_system":"Antioxidant pretreatment of engineered HEK293/Pank1beta cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-coalation2017] Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. 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