{"id":"85457949-9b80-593e-bfb6-3b5553db03ef","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-copper-pde3b","predicate":"inhibits","statement":"Copper inhibited mouse PDE3B activity in the biochemical and adipocyte study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6e6c4eae-3951-5a58-bb8d-ff4245903f6e","mechanism_event_label":"Copper slowed an enzyme that normally clears the cAMP signal.","subject":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"object":{"id":"76a46ee5-623f-558f-be84-327fb74dfb08","slug":"mouse-pde3b","display_name":"Mouse cGMP-inhibited phosphodiesterase Pde3b","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"6e6c4eae-3951-5a58-bb8d-ff4245903f6e","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-copper-pde3b-event","event_type":"biochemical_relationship","label":"Copper slowed an enzyme that normally clears the cAMP signal.","description":"Copper inhibited mouse PDE3B activity in the biochemical and adipocyte study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"76a46ee5-623f-558f-be84-327fb74dfb08","slug":"mouse-pde3b","display_name":"Mouse cGMP-inhibited phosphodiesterase Pde3b","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/copper-research/27272565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\", \"start_char\": 0, \"end_char\": 1018, \"text_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Copper manipulation, purified Pde3b assays and adipocyte signaling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Copper perturbation and Pde3b cysteine mutations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This cellular signaling result is not a human weight-loss trial. Copper toxicity and systemic distribution are not captured by increasing one cell-culture signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Copper research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse proteins and 3T3-L1 adipocytes; Atp7b mutant mouse context","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Copper slowed an enzyme that normally clears the cAMP signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[copper-p27272565] Copper regulates cyclic-AMP-dependent lipolysis. (2016). https://pubmed.ncbi.nlm.nih.gov/27272565/ DOI: 10.1038/nchembio.2098","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adipocytes and purified enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ca81e083-1d48-562d-b16b-4d769b91d295","evidence_kind":"source_excerpt","locator":"Lines 1196-1207","start_line":1196,"end_line":1207,"excerpt":"### copper-copper-pde3b\nCopper inhibited mouse PDE3B activity in the biochemical and adipocyte study.\nCondition category: normal\nnutrient_topic: Copper research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Copper slowed an enzyme that normally clears the cAMP signal.\norganism: Mouse proteins and 3T3-L1 adipocytes; Atp7b mutant mouse context\ntissue_or_cell_type: Adipocytes and purified enzyme\nexperimental_model: Copper manipulation, purified Pde3b assays and adipocyte signaling\nlimitations: This cellular signaling result is not a human weight-loss trial. Copper toxicity and systemic distribution are not captured by increasing one cell-culture signal.\nexposure: Copper perturbation and Pde3b cysteine mutations\nevidence_span: {\"source_cache\": \"artifacts/copper-research/27272565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\", \"start_char\": 0, \"end_char\": 1018, \"text_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\"}\n[copper-p27272565] Copper regulates cyclic-AMP-dependent lipolysis. (2016). https://pubmed.ncbi.nlm.nih.gov/27272565/ DOI: 10.1038/nchembio.2098","model_system":"Copper manipulation, purified Pde3b assays and adipocyte signaling","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [copper-p27272565] Copper regulates cyclic-AMP-dependent lipolysis. (2016). https://pubmed.ncbi.nlm.nih.gov/27272565/ DOI: 10.1038/nchembio.2098","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9afba495-cbdc-51aa-998e-70a930dba3be","stable_key":"import-0ad8610d-d575-5870-b7cd-763a9f750783","title":"Copper: transport, cuproenzymes, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"84b0f62b2dae6835fa26902be87625c003c6c707492d3007e8f9d15420669008","revision_id":"d7e35b8b-3f77-56d9-90b5-5f542c63f321","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}