{"id":"84dc3d6f-82e9-5ba1-bc4f-26026b35d7f1","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-copper-complex","predicate":"reported_relationship","statement":"Apigenin formed Cu(II) complexes in the cell-free metal-binding study despite lacking a catechol group.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"864b3300-d604-5c04-8ea0-24ebe2aa049b","mechanism_event_label":"Apigenin formed Cu(II) complexes in the cell-free metal-binding study despite lacking a catechol group.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"864b3300-d604-5c04-8ea0-24ebe2aa049b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-copper-complex-event","event_type":"biochemical_relationship","label":"Apigenin formed Cu(II) complexes in the cell-free metal-binding study despite lacking a catechol group.","description":"### api-copper-complex\n\nApigenin formed Cu(II) complexes in the cell-free metal-binding study despite lacking a catechol group.\nCoordination involving the 5-hydroxyl/4-oxo region was proposed. Complex formation does not establish therapeutic chelation, copper depletion or net antioxidant benefit.\nModel and exposure: UV spectroscopy and electrospray mass spectrometry; pH 5.5 and 7.4.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. | 2002 | https://pubmed.ncbi.nlm.nih.gov/12592672/ | https://doi.org/10.1080/1071576021000016463 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"UV spectroscopy and electrospray mass spectrometry; pH 5.5 and 7.4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin formed Cu(II) complexes in the cell-free metal-binding study despite lacking a catechol group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. | https://pubmed.ncbi.nlm.nih.gov/12592672/ | https://doi.org/10.1080/1071576021000016463","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-copper-complex; Coordination involving the 5-hydroxyl/4-oxo region was proposed. Complex formation does not establish therapeutic chelation, copper depletion or net antioxidant benefit.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"605afcdb-851c-5f19-be7e-2e0773b0622b","evidence_kind":"source_excerpt","locator":"Lines 912-918","start_line":912,"end_line":918,"excerpt":"### api-copper-complex\n\nApigenin formed Cu(II) complexes in the cell-free metal-binding study despite lacking a catechol group.\nCoordination involving the 5-hydroxyl/4-oxo region was proposed. Complex formation does not establish therapeutic chelation, copper depletion or net antioxidant benefit.\nModel and exposure: UV spectroscopy and electrospray mass spectrometry; pH 5.5 and 7.4.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. | 2002 | https://pubmed.ncbi.nlm.nih.gov/12592672/ | https://doi.org/10.1080/1071576021000016463 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"UV spectroscopy and electrospray mass spectrometry; pH 5.5 and 7.4.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}