{"id":"84a853db-2aee-5c75-a039-c4780e16671b","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-memory-cytokines","predicate":"restores","statement":"Alanine add-back restored IFN-gamma and TNF production suppressed during five-hour antigen restimulation of mouse memory CD8 T cells in dialyzed-serum medium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4ee8cf3a-a1fd-5ced-b985-8bc684dfbc9b","mechanism_event_label":"Memory cells needed available alanine to restart these cytokine responses.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"e0226621-7ba6-5ffc-a462-d9496cb2a25d","slug":"mouse-memory-t-cell-ifng-tnf","display_name":"IFN-gamma and TNF output after mouse memory T-cell restimulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4ee8cf3a-a1fd-5ced-b985-8bc684dfbc9b","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-memory-cytokines-event","event_type":"observed_relationship","label":"Memory cells needed available alanine to restart these cytokine responses.","description":"Alanine add-back restored IFN-gamma and TNF production suppressed during five-hour antigen restimulation of mouse memory CD8 T cells in dialyzed-serum medium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e0226621-7ba6-5ffc-a462-d9496cb2a25d","slug":"mouse-memory-t-cell-ifng-tnf","display_name":"IFN-gamma and TNF output after mouse memory T-cell restimulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"P14/LCMV mouse model; day-30 memory splenocytes, GP33–41 peptide ex vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Granzyme B was not similarly reduced; do not describe every immune function as alanine-dependent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Memory cells needed available alanine to restart these cytokine responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"T Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"be28b542-b057-54ed-9210-2b945835d9dc","evidence_kind":"source_excerpt","locator":"Lines 120-126","start_line":120,"end_line":126,"excerpt":"## alanine-memory-cytokines\nMemory cells needed available alanine to restart these cytokine responses.\nAlanine add-back restored IFN-gamma and TNF production suppressed during five-hour antigen restimulation of mouse memory CD8 T cells in dialyzed-serum medium.\nModel: P14/LCMV mouse model; day-30 memory splenocytes, GP33–41 peptide ex vivo.\nLimitations: Granzyme B was not similarly reduced; do not describe every immune function as alanine-dependent.\nEvidence access: Primary full text\nT Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","model_system":"P14/LCMV mouse model; day-30 memory splenocytes, GP33–41 peptide ex vivo.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}