{"id":"84942c39-c562-51af-9c23-61a83c9fe913","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:aldh1l2-targeting","predicate":"localizes-to","statement":"A human ALDH1L2 construct carrying its N-terminal targeting region localized to mitochondria in transfected cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b0abc12c-64b8-51fd-93be-0ca078fb85b4","mechanism_event_label":"ALDH1L2 is the mitochondrial counterpart of cytosolic ALDH1L1.","subject":{"id":"8079aee1-6515-5733-80fa-e243016e3b4a","slug":"aldh1l2","display_name":"Human ALDH1L2","entity_type_key":"protein"},"object":{"id":"f5bd4edd-4298-5985-976f-897fd415c3d5","slug":"mitochondria","display_name":"Mitochondria","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"b0abc12c-64b8-51fd-93be-0ca078fb85b4","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:aldh1l2-targeting-event","event_type":"observed_intervention","label":"ALDH1L2 is the mitochondrial counterpart of cytosolic ALDH1L1.","description":"A human ALDH1L2 construct carrying its N-terminal targeting region localized to mitochondria in transfected cells.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"8079aee1-6515-5733-80fa-e243016e3b4a","slug":"aldh1l2","display_name":"Human ALDH1L2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f5bd4edd-4298-5985-976f-897fd415c3d5","slug":"mitochondria","display_name":"Mitochondria","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Fluorescent protein localization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Localization alone does not measure carbon-disposal flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ALDH1L2 is the mitochondrial counterpart of cytosolic ALDH1L1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[krupenko-2010] ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase (2010). https://pubmed.ncbi.nlm.nih.gov/20498374/ DOI: 10.1074/jbc.m110.128843","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"A549 cells; corroborating COS-7 host experiments","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9a482935-476c-5c37-b167-4914f3333fb9","evidence_kind":"source_excerpt","locator":"Lines 1034-1044","start_line":1034,"end_line":1044,"excerpt":"### aldh1l2-targeting\nA human ALDH1L2 construct carrying its N-terminal targeting region localized to mitochondria in transfected cells.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ALDH1L2 is the mitochondrial counterpart of cytosolic ALDH1L1.\norganism: Homo sapiens\ntissue_or_cell_type: A549 cells; corroborating COS-7 host experiments\nexperimental_model: Fluorescent protein localization\nlimitations: Localization alone does not measure carbon-disposal flux.\nexposure: Assay conditions described in the linked primary study.\n[krupenko-2010] ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase (2010). https://pubmed.ncbi.nlm.nih.gov/20498374/ DOI: 10.1074/jbc.m110.128843","model_system":"Fluorescent protein localization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [krupenko-2010] ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase (2010). https://pubmed.ncbi.nlm.nih.gov/20498374/ DOI: 10.1074/jbc.m110.128843","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}