{"id":"848f8e25-f616-5998-a1ea-829797fcc7c8","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-behavior-challenge","predicate":"elicits_selected_responder_change","statement":"The blinded challenge identified dose-related irritability, restlessness or sleep disturbance in a subset of the selected children.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"570fd708-371d-5a37-bcd1-1a0b097524b9","mechanism_event_label":"The blinded challenge identified dose-related irritability, restlessness or sleep disturbance in a subset of the selected children.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"d1db25b6-9027-5479-a965-be6d3c900047","slug":"human-tartrazine-challenge-behavior","display_name":"Parent-rated behavior after blinded tartrazine challenge in selected children","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"570fd708-371d-5a37-bcd1-1a0b097524b9","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-behavior-challenge-event","event_type":"observed_relationship","label":"The blinded challenge identified dose-related irritability, restlessness or sleep disturbance in a subset of the selected children.","description":"The blinded challenge identified dose-related irritability, restlessness or sleep disturbance in a subset of the selected children.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d1db25b6-9027-5479-a965-be6d3c900047","slug":"human-tartrazine-challenge-behavior","display_name":"Parent-rated behavior after blinded tartrazine challenge in selected children","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Placebo or tartrazine 1, 2, 5, 10, 20 or 50 mg each challenge morning","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"21-day repeated-measures study; ratings for each 24 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Selected children aged 2-14 years: 34 suspected/uncertain reactors and 20 controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Recruitment enriched for suspected reactions; parent ratings and individual-response classification do not prove universal effects or a mechanism mediated by zinc.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Selected children aged 2-14 years: 34 suspected/uncertain reactors and 20 controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The blinded challenge identified dose-related irritability, restlessness or sleep disturbance in a subset of the selected children.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Synthetic food coloring and behavior: a dose response effect in a double-blind, placebo-controlled, repeated-measures study. (1994). https://pubmed.ncbi.nlm.nih.gov/7965420/ DOI: 10.1016/s0022-3476(94)70059-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Oral administration","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Blinded parent-rated behavior","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2d691476-82d4-5c22-9f0f-0deb8fea1716","evidence_kind":"source_excerpt","locator":"Lines 314-323","start_line":314,"end_line":323,"excerpt":"## tartrazine-behavior-challenge\nThe blinded challenge identified dose-related irritability, restlessness or sleep disturbance in a subset of the selected children.\nModel/species: Selected children aged 2-14 years: 34 suspected/uncertain reactors and 20 controls\nTissue: Blinded parent-rated behavior\nExposure: Placebo or tartrazine 1, 2, 5, 10, 20 or 50 mg each challenge morning\nRoute: Oral administration\nDuration: 21-day repeated-measures study; ratings for each 24 h\nLimits: Recruitment enriched for suspected reactions; parent ratings and individual-response classification do not prove universal effects or a mechanism mediated by zinc.\nPrimary reference: Synthetic food coloring and behavior: a dose response effect in a double-blind, placebo-controlled, repeated-measures study. (1994). https://pubmed.ncbi.nlm.nih.gov/7965420/ DOI: 10.1016/s0022-3476(94)70059-1\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Selected children aged 2-14 years: 34 suspected/uncertain reactors and 20 controls","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. 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