{"id":"84825001-a8f0-583f-b461-7646e7ac647b","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:digestion-releases-ace-inhibitory-peptides-from-nattokinase","predicate":"is_digested_to","statement":"Heat fragmentation and simulated gastrointestinal digestion both release ACE-inhibitory peptides from the nattokinase sequence.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"67a89385-8f5b-5082-ae76-16ffd8df40ea","mechanism_event_label":"Heat fragmentation and simulated gastrointestinal digestion both release ACE-inhibitory peptides from the nattokinase sequence.","subject":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"object":{"id":"81a46755-4d84-5aa8-9411-a83bae3a2887","slug":"nattokinase-digestion-peptides","display_name":"Nattokinase-derived digestion peptide pool","entity_type_key":"peptide"},"evidence_count":1,"mechanism_event":{"id":"67a89385-8f5b-5082-ae76-16ffd8df40ea","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:digestion-releases-ace-inhibitory-peptides-from-nattokinase-event","event_type":"biochemical_relationship","label":"Heat fragmentation and simulated gastrointestinal digestion both release ACE-inhibitory peptides from the nattokinase sequence.","description":"Simulated digestion with peptide identification by HPLC-MS","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"81a46755-4d84-5aa8-9411-a83bae3a2887","slug":"nattokinase-digestion-peptides","display_name":"Nattokinase-derived digestion peptide pool","entity_type_key":"peptide"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified nattokinase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Nattokinase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro generation. Whether these peptides are generated in a human gut, absorbed, or reach concentrations that inhibit ACE in vivo has not been measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Purified nattokinase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Heat fragmentation and simulated gastrointestinal digestion both release ACE-inhibitory peptides from the nattokinase sequence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification and mechanistic characterization of novel ACE-inhibitory peptides derived from Nattokinase. 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Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. 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