{"id":"8470e166-568c-5bec-910c-2a23bdc4ce71","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-beta-current","predicate":"produces","statement":"Arginine generated an inward current carried by the amino acid; CAT2A-mediated electrogenic uptake was the proposed route.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"52f90d8d-14bb-5e29-81e1-f20c0842c393","mechanism_event_label":"Moving a positively charged amino acid can change membrane voltage.","subject":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"object":{"id":"27e7e5d5-f617-59f8-a698-b768cab321fb","slug":"mouse-beta-cell-inward-current","display_name":"Arginine-carried inward current in mouse beta cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"52f90d8d-14bb-5e29-81e1-f20c0842c393","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-beta-current-event","event_type":"observed_relationship","label":"Moving a positively charged amino acid can change membrane voltage.","description":"Arginine generated an inward current carried by the amino acid; CAT2A-mediated electrogenic uptake was the proposed route.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"27e7e5d5-f617-59f8-a698-b768cab321fb","slug":"mouse-beta-cell-inward-current","display_name":"Arginine-carried inward current in mouse beta cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ba0bf1ab-cc35-5761-acaa-82186c86f16d","slug":"mouse-slc7a2-cat2a","display_name":"Mouse cationic amino acid transporter CAT2A / Slc7a2 isoform","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"450ebb9e-ee95-57cd-bfe1-65ee7599164f","slug":"mouse-beta-cell-depolarization","display_name":"Mouse pancreatic beta-cell membrane depolarization","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell/animal mechanism; no universal human insulin response inferred. NAD(P)H autofluorescence did not show increased metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Moving a positively charged amino acid can change membrane voltage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Electrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9130159/ · DOI 10.1113/jphysiol.1997.sp021955","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"43c4ea04-64b3-5e8a-9c79-435556061f8a","evidence_kind":"source_excerpt","locator":"Lines 302-308","start_line":302,"end_line":308,"excerpt":"## arg-beta-current\nMoving a positively charged amino acid can change membrane voltage.\nArginine generated an inward current carried by the amino acid; CAT2A-mediated electrogenic uptake was the proposed route.\nModel: Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.\nLimitations: Cell/animal mechanism; no universal human insulin response inferred. NAD(P)H autofluorescence did not show increased metabolism.\nEvidence access: Primary abstract\nElectrogenic arginine transport mediates stimulus-secretion coupling in mouse pancreatic beta-cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9130159/ · DOI 10.1113/jphysiol.1997.sp021955","model_system":"Mouse pancreatic beta cells; electrophysiology, calcium imaging and secretion experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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