{"id":"842a56d7-f8ab-539e-b118-4fd38604caf3","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-nfs1-cytosol","predicate":"interacts_in_cytosol_with","statement":"FRET and split-EGFP supported an NFS1-MOCS3 interaction in the cytosol of human cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d4d1253f-af26-58d3-b563-77713d51dd4e","mechanism_event_label":"Sulfur delivery to the molybdenum pathway also occurs outside mitochondria.","subject":{"id":"9838e184-216a-5a26-a8c5-1777b9dd2d90","slug":"nfs1","display_name":"Human cysteine desulfurase / NFS1","entity_type_key":"protein"},"object":{"id":"c9f482f8-899b-5a59-9745-05a8709d91a2","slug":"mocs3","display_name":"Human adenylyltransferase and sulfurtransferase / MOCS3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d4d1253f-af26-58d3-b563-77713d51dd4e","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-nfs1-cytosol-event","event_type":"biochemical_relationship","label":"Sulfur delivery to the molybdenum pathway also occurs outside mitochondria.","description":"FRET and split-EGFP supported an NFS1-MOCS3 interaction in the cytosol of human cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9838e184-216a-5a26-a8c5-1777b9dd2d90","slug":"nfs1","display_name":"Human cysteine desulfurase / NFS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c9f482f8-899b-5a59-9745-05a8709d91a2","slug":"mocs3","display_name":"Human adenylyltransferase and sulfurtransferase / MOCS3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/23593335.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"de38fd3acf5b473459161bd9fc6bde8a7d13d8734336d445f36938684e50711b\", \"start_char\": 0, \"end_char\": 1050, \"text_sha256\": \"de38fd3acf5b473459161bd9fc6bde8a7d13d8734336d445f36938684e50711b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HeLa localization/FRET and split-EGFP; purified protein complementation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cell fractionation, immunodetection and protein interaction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cytosolic NFS1 is distinct from assuming all NFS1 sulfur transfer happens inside mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens; separate Neurospora crassa complementation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sulfur delivery to the molybdenum pathway also occurs outside mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p23593335] The L-cysteine desulfurase NFS1 is localized in the cytosol where it provides the sulfur for molybdenum cofactor biosynthesis in humans. (2013). https://pubmed.ncbi.nlm.nih.gov/23593335/ DOI: 10.1371/journal.pone.0060869","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human cytosol and purified proteins","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7a04ef6e-a7bd-5708-a70e-d89b2de03420","evidence_kind":"source_excerpt","locator":"Lines 378-389","start_line":378,"end_line":389,"excerpt":"### mo-nfs1-cytosol\nFRET and split-EGFP supported an NFS1-MOCS3 interaction in the cytosol of human cells.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Sulfur delivery to the molybdenum pathway also occurs outside mitochondria.\norganism: Homo sapiens; separate Neurospora crassa complementation\ntissue_or_cell_type: Human cytosol and purified proteins\nexperimental_model: HeLa localization/FRET and split-EGFP; purified protein complementation\nlimitations: Cytosolic NFS1 is distinct from assuming all NFS1 sulfur transfer happens inside mitochondria.\nexposure: Cell fractionation, immunodetection and protein interaction\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/23593335.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"de38fd3acf5b473459161bd9fc6bde8a7d13d8734336d445f36938684e50711b\", \"start_char\": 0, \"end_char\": 1050, \"text_sha256\": \"de38fd3acf5b473459161bd9fc6bde8a7d13d8734336d445f36938684e50711b\"}\n[mo-p23593335] The L-cysteine desulfurase NFS1 is localized in the cytosol where it provides the sulfur for molybdenum cofactor biosynthesis in humans. (2013). https://pubmed.ncbi.nlm.nih.gov/23593335/ DOI: 10.1371/journal.pone.0060869","model_system":"HeLa localization/FRET and split-EGFP; purified protein complementation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p23593335] The L-cysteine desulfurase NFS1 is localized in the cytosol where it provides the sulfur for molybdenum cofactor biosynthesis in humans. (2013). https://pubmed.ncbi.nlm.nih.gov/23593335/ DOI: 10.1371/journal.pone.0060869","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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