{"id":"8416583e-2066-57a1-8fc4-691ea438cfff","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-rabbit-phosphorylase-plp","predicate":"binds-to","statement":"Rabbit muscle phosphorylase crystallography located PLP buried next to the catalytic glucose-binding site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d146e95f-707e-5179-952e-09560f6af675","mechanism_event_label":"B6 also participates in glycogen metabolism.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"5a1cc7d4-ac45-5843-9f0a-a13b051fb686","slug":"rabbit-pygm","display_name":"Rabbit skeletal-muscle glycogen phosphorylase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d146e95f-707e-5179-952e-09560f6af675","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-rabbit-phosphorylase-plp-event","event_type":"biochemical_relationship","label":"B6 also participates in glycogen metabolism.","description":"Rabbit muscle phosphorylase crystallography located PLP buried next to the catalytic glucose-binding site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5a1cc7d4-ac45-5843-9f0a-a13b051fb686","slug":"rabbit-pygm","display_name":"Rabbit skeletal-muscle glycogen phosphorylase","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4c77dafc-3257-5fca-8d67-6d7672a11351","slug":"glucose-1-phosphate","display_name":"alpha-D-Glucose 1-phosphate","entity_type_key":"small_molecule"},"role":"tested substrate/product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B6-linked glycogen chemistry involves phosphate-containing substrates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rabbit skeletal muscle glycogen phosphorylase crystallography","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rabbit structure; the spatial relationship alone does not establish every catalytic step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"B6 also participates in glycogen metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-phosphorylase-1977] Location of pyridoxal phosphate in glycogen phosphorylase a. (1977). https://pmc.ncbi.nlm.nih.gov/articles/PMC432034/ DOI: 10.1073/pnas.74.11.4757","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rabbit skeletal-muscle protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7c826dde-831d-5f38-9692-3cb6a14d0503","evidence_kind":"source_excerpt","locator":"Lines 848-858","start_line":848,"end_line":858,"excerpt":"### b6-met-rabbit-phosphorylase-plp\nRabbit muscle phosphorylase crystallography located PLP buried next to the catalytic glucose-binding site.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: B6 also participates in glycogen metabolism.\norganism: Oryctolagus cuniculus\ntissue_or_cell_type: Rabbit skeletal-muscle protein\nexperimental_model: Rabbit skeletal muscle glycogen phosphorylase crystallography\nlimitations: Rabbit structure; the spatial relationship alone does not establish every catalytic step.\ncross_nutrient: B6-linked glycogen chemistry involves phosphate-containing substrates.\n[b6-phosphorylase-1977] Location of pyridoxal phosphate in glycogen phosphorylase a. (1977). https://pmc.ncbi.nlm.nih.gov/articles/PMC432034/ DOI: 10.1073/pnas.74.11.4757","model_system":"Rabbit skeletal muscle glycogen phosphorylase crystallography","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-phosphorylase-1977] Location of pyridoxal phosphate in glycogen phosphorylase a. (1977). https://pmc.ncbi.nlm.nih.gov/articles/PMC432034/ DOI: 10.1073/pnas.74.11.4757","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}