{"id":"8397ae1e-9be7-5fc1-bf5f-088660a33591","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-smit2-glucose","predicate":"competitively_inhibits","statement":"Glucose competed for D-chiro-inositol uptake with a reported Ki of 6.1 mM in the SMIT2 expression system, without evidence that SMIT2 transported glucose in those assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"796b3778-8403-5c79-9303-d86b1e961aef","mechanism_event_label":"Glucose competed for entry without itself becoming demonstrated cargo of this transporter.","subject":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"object":{"id":"80a5a753-57c3-5394-b464-e67f1347af53","slug":"dci-uptake","display_name":"D-chiro-inositol uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"796b3778-8403-5c79-9303-d86b1e961aef","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-smit2-glucose-event","event_type":"biochemical_relationship","label":"Glucose competed for entry without itself becoming demonstrated cargo of this transporter.","description":"Glucose competed for D-chiro-inositol uptake with a reported Ki of 6.1 mM in the SMIT2 expression system, without evidence that SMIT2 transported glucose in those assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f1ba9418-ff0d-5aea-ac90-c97614cc4150","slug":"slc5a11","display_name":"SLC5A11 (human SMIT2)","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80a5a753-57c3-5394-b464-e67f1347af53","slug":"dci-uptake","display_name":"D-chiro-inositol uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/19032932.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4fdc565b9aa26ef5f2d083c539a1b0367403b39f0c75d3ea1b194bf6fa525242\", \"start_char\": 0, \"end_char\": 1164, \"text_sha256\": \"4fdc565b9aa26ef5f2d083c539a1b0367403b39f0c75d3ea1b194bf6fa525242\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SMIT2 overexpression and radiotracer uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Overexpression, glucose competition and insulin exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Overexpression is not normal transporter abundance. Insulin experiments used untransfected rat cells; changes in human diabetes are proposed, not demonstrated here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human transporter in rat L6 myoblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glucose competed for entry without itself becoming demonstrated cargo of this transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p19032932] Human sodium/inositol cotransporter 2 (SMIT2) transports inositols but not glucose in L6 cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19032932/ DOI: 10.1016/j.abb.2008.11.008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal-muscle cell model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"95a447f1-e7da-5a24-afef-9f5f091c58f3","evidence_kind":"source_excerpt","locator":"Lines 314-325","start_line":314,"end_line":325,"excerpt":"### ino-smit2-glucose\nGlucose competed for D-chiro-inositol uptake with a reported Ki of 6.1 mM in the SMIT2 expression system, without evidence that SMIT2 transported glucose in those assays.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Glucose competed for entry without itself becoming demonstrated cargo of this transporter.\norganism: Human transporter in rat L6 myoblasts\ntissue_or_cell_type: Skeletal-muscle cell model\nexperimental_model: Human SMIT2 overexpression and radiotracer uptake\nlimitations: Overexpression is not normal transporter abundance. Insulin experiments used untransfected rat cells; changes in human diabetes are proposed, not demonstrated here.\nexposure: Overexpression, glucose competition and insulin exposure\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/19032932.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4fdc565b9aa26ef5f2d083c539a1b0367403b39f0c75d3ea1b194bf6fa525242\", \"start_char\": 0, \"end_char\": 1164, \"text_sha256\": \"4fdc565b9aa26ef5f2d083c539a1b0367403b39f0c75d3ea1b194bf6fa525242\"}\n[ino-p19032932] Human sodium/inositol cotransporter 2 (SMIT2) transports inositols but not glucose in L6 cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19032932/ DOI: 10.1016/j.abb.2008.11.008","model_system":"Human SMIT2 overexpression and radiotracer uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p19032932] Human sodium/inositol cotransporter 2 (SMIT2) transports inositols but not glucose in L6 cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19032932/ DOI: 10.1016/j.abb.2008.11.008","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}