{"id":"83313ebf-375e-51f1-875c-be7ac86df4b1","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-mpo-hocl","predicate":"oxidizes_chloride_to","statement":"Myeloperoxidase used hydrogen peroxide to oxidize chloride to hypochlorous acid.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fb5e81df-e6c0-5c9a-a40c-2fa40f7bd187","mechanism_event_label":"Immune chemistry can convert chloride into a reactive antimicrobial oxidant.","subject":{"id":"53f51828-4ea1-5f2e-a044-e02577272436","slug":"mpo","display_name":"Human myeloperoxidase / MPO","entity_type_key":"protein"},"object":{"id":"9bb71c3b-47d9-52bd-93ec-f2b06b3355a3","slug":"hypochlorous-acid","display_name":"Hypochlorous acid / HOCl","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"fb5e81df-e6c0-5c9a-a40c-2fa40f7bd187","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-mpo-hocl-event","event_type":"biochemical_relationship","label":"Immune chemistry can convert chloride into a reactive antimicrobial oxidant.","description":"Myeloperoxidase used hydrogen peroxide to oxidize chloride to hypochlorous acid.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"oxidant","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53f51828-4ea1-5f2e-a044-e02577272436","slug":"mpo","display_name":"Human myeloperoxidase / MPO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9bb71c3b-47d9-52bd-93ec-f2b06b3355a3","slug":"hypochlorous-acid","display_name":"Hypochlorous acid / HOCl","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/9359420.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\", \"start_char\": 0, \"end_char\": 1700, \"text_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified-enzyme substrate kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 mM chloride with varying thiocyanate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Product chemistry under assay conditions; not a recommendation to raise chloride or thiocyanate intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human neutrophil enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Immune chemistry can convert chloride into a reactive antimicrobial oxidant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p9359420] Thiocyanate and chloride as competing substrates for myeloperoxidase. (1997). https://pubmed.ncbi.nlm.nih.gov/9359420/ DOI: 10.1042/bj3270487","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Myeloperoxidase reaction mixture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ca212d5d-7689-58f2-83bd-0cbcddd4e0b5","evidence_kind":"source_excerpt","locator":"Lines 614-625","start_line":614,"end_line":625,"excerpt":"### chloride-mpo-hocl\nMyeloperoxidase used hydrogen peroxide to oxidize chloride to hypochlorous acid.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Immune chemistry can convert chloride into a reactive antimicrobial oxidant.\norganism: Human neutrophil enzyme\ntissue_or_cell_type: Myeloperoxidase reaction mixture\nexperimental_model: Purified-enzyme substrate kinetics\nlimitations: Product chemistry under assay conditions; not a recommendation to raise chloride or thiocyanate intake.\nexposure: 100 mM chloride with varying thiocyanate\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/9359420.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\", \"start_char\": 0, \"end_char\": 1700, \"text_sha256\": \"83dc6a264dfb45201104568ac58c76509aaa0fabca1f32efd25861146b4b38c0\"}\n[chloride-p9359420] Thiocyanate and chloride as competing substrates for myeloperoxidase. 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