{"id":"82a5c091-6d2d-5531-b401-b8734473a501","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-repair-depletion","predicate":"inhibition_impairs","statement":"FK866-mediated NAD depletion impaired DNA repair and damage-induced PARylation in the tested human cancer-cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"885e420d-102a-5c7f-8e29-4607ff311176","mechanism_event_label":"When salvage is blocked, repair enzymes can lose access to their substrate.","subject":{"id":"8272125c-0d87-5920-9dae-7409b797bbfa","slug":"nampt","display_name":"Human nicotinamide phosphoribosyltransferase / NAMPT","entity_type_key":"protein"},"object":{"id":"91fc54fc-a99b-544e-ba84-cce1efb66823","slug":"human-mcf7-dna-repair-after-nad-loss","display_name":"DNA repair after NAD depletion in human MCF-7 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"885e420d-102a-5c7f-8e29-4607ff311176","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-repair-depletion-event","event_type":"observed_relationship","label":"When salvage is blocked, repair enzymes can lose access to their substrate.","description":"FK866-mediated NAD depletion impaired DNA repair and damage-induced PARylation in the tested human cancer-cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8272125c-0d87-5920-9dae-7409b797bbfa","slug":"nampt","display_name":"Human nicotinamide phosphoribosyltransferase / NAMPT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"91fc54fc-a99b-544e-ba84-cce1efb66823","slug":"human-mcf7-dna-repair-after-nad-loss","display_name":"DNA repair after NAD depletion in human MCF-7 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d5ab002d-61f0-502f-951a-00b96f059988","slug":"parp1","display_name":"Human PARP1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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