{"id":"82880ec1-2608-5081-924b-221f022689d6","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-adams-d2-serum-rescue","predicate":"improves-serum-supported-ex-vivo-response","statement":"Serum collected after D2 treatment of low-status patients better supported TLR-triggered monocyte hCAP expression ex vivo.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1dd43aee-809c-59d4-af4f-1ec27fcf4fcd","mechanism_event_label":"D2 treatment changed serum support for a local cell response.","subject":{"id":"6e1cf31c-f78e-596b-b1b3-70585cf9c948","slug":"ergocalciferol","display_name":"Ergocalciferol / vitamin D2","entity_type_key":"small_molecule"},"object":{"id":"24a49e8d-7865-5e47-92a0-0ffadd48cb24","slug":"human-camp-mrna","display_name":"Human CAMP mRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"1dd43aee-809c-59d4-af4f-1ec27fcf4fcd","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-adams-d2-serum-rescue-event","event_type":"observed_intervention","label":"D2 treatment changed serum support for a local cell response.","description":"Serum collected after D2 treatment of low-status patients better supported TLR-triggered monocyte hCAP expression ex vivo.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"role":"related-precursor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"92118f85-ffc4-5f77-bfca-4b033b001548","slug":"25-hydroxyvitamin-d2","display_name":"25-Hydroxyvitamin D2","entity_type_key":"small_molecule"},"role":"D2-derived-precursor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e1cf31c-f78e-596b-b1b3-70585cf9c948","slug":"ergocalciferol","display_name":"Ergocalciferol / vitamin D2","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"24a49e8d-7865-5e47-92a0-0ffadd48cb24","slug":"human-camp-mrna","display_name":"Human CAMP mRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"D2 50, 000 IU twice weekly 5 weeks in low-status patients; paired serum used for ex-vivo assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Before/after serum comparison; no randomized D2/D3 head-to-head or demonstrated reduction of infections.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"D2 treatment changed serum support for a local cell response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. (2009). https://pubmed.ncbi.nlm.nih.gov/19299728/ DOI: 10.4049/jimmunol.0803736","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Patient serum and ex-vivo monocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f974edf2-3159-50b4-893f-ac85415e27c6","evidence_kind":"source_excerpt","locator":"Lines 1009-1020","start_line":1009,"end_line":1020,"excerpt":"### vd-adams-d2-serum-rescue\nSerum collected after D2 treatment of low-status patients better supported TLR-triggered monocyte hCAP expression ex vivo.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: D2 treatment changed serum support for a local cell response.\norganism: Homo sapiens\ntissue_or_cell_type: Patient serum and ex-vivo monocytes\nexperimental_model: Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum\nlimitations: Before/after serum comparison; no randomized D2/D3 head-to-head or demonstrated reduction of infections.\nexposure: D2 50, 000 IU twice weekly 5 weeks in low-status patients; paired serum used for ex-vivo assays.\ncross_nutrient: false\n[adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. 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