{"id":"8277e212-6416-51f2-b884-98824d38c7e7","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-eanb-mechanism","predicate":"sulfurizes","statement":"Structural and kinetic analyses of Chlorobium limicola EanB supported sulfurization of hercynine in anaerobic ergothioneine synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ae5cb5e9-f091-5f38-b7e5-882be32298dd","mechanism_event_label":"Different machinery can produce the same compound.","subject":{"id":"240f8d81-953c-5f7c-8d0a-411477ed886c","slug":"chlorobium-limicola-eanb","display_name":"Chlorobium limicola EanB","entity_type_key":"protein"},"object":{"id":"b3583649-cbbd-50f1-be33-26b1ee0dd6ab","slug":"hercynine","display_name":"Hercynine / N-alpha-trimethylhistidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ae5cb5e9-f091-5f38-b7e5-882be32298dd","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-eanb-mechanism-event","event_type":"observed_relationship","label":"Different machinery can produce the same compound.","description":"Structural and kinetic analyses of Chlorobium limicola EanB supported sulfurization of hercynine in anaerobic ergothioneine synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"240f8d81-953c-5f7c-8d0a-411477ed886c","slug":"chlorobium-limicola-eanb","display_name":"Chlorobium limicola EanB","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b3583649-cbbd-50f1-be33-26b1ee0dd6ab","slug":"hercynine","display_name":"Hercynine / N-alpha-trimethylhistidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacterial enzyme crystal structure and kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not project this microbial enzyme onto human tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Different machinery can produce the same compound.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural and Mechanistic Basis for Anaerobic Ergothioneine Biosynthesis. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30943021/ · DOI 10.1021/jacs.8b12596","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7e652584-0c9f-552e-9884-4c4758b7b67e","evidence_kind":"source_excerpt","locator":"Lines 224-230","start_line":224,"end_line":230,"excerpt":"## ergothioneine-eanb-mechanism\nDifferent machinery can produce the same compound.\nStructural and kinetic analyses of Chlorobium limicola EanB supported sulfurization of hercynine in anaerobic ergothioneine synthesis.\nModel: Bacterial enzyme crystal structure and kinetics.\nLimitations: Do not project this microbial enzyme onto human tissues.\nEvidence access: Primary abstract\nStructural and Mechanistic Basis for Anaerobic Ergothioneine Biosynthesis. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30943021/ · DOI 10.1021/jacs.8b12596","model_system":"Bacterial enzyme crystal structure and kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}