{"id":"82063758-7f2a-5a17-9c23-5fb57ade5536","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-lat1-subunit","predicate":"supports","statement":"Purified human LAT1 alone transported histidine in proteoliposomes; purified CD98 alone did not.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"08ac3dba-ab46-5432-8a4b-404cf264803e","mechanism_event_label":"The transport pore and its partnering protein have distinct roles.","subject":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"object":{"id":"d89c30e9-622c-542a-a903-dd80d1f0be0e","slug":"human-lat1-transport-competence","display_name":"Transport competence of reconstituted human LAT1","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"08ac3dba-ab46-5432-8a4b-404cf264803e","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-lat1-subunit-event","event_type":"observed_relationship","label":"The transport pore and its partnering protein have distinct roles.","description":"Purified human LAT1 alone transported histidine in proteoliposomes; purified CD98 alone did not.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d89c30e9-622c-542a-a903-dd80d1f0be0e","slug":"human-lat1-transport-competence","display_name":"Transport competence of reconstituted human LAT1","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1ca4b4d5-616a-54d3-8244-0dc151b1bcfe","slug":"slc3a2","display_name":"4F2hc / SLC3A2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human LAT1 and CD98 reconstitution.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"CD98 dispensability for this assay does not mean dispensability for membrane targeting in intact tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Histidine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The transport pore and its partnering protein have distinct roles.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"LAT1 is the transport competent unit of the LAT1/CD98 heterodimeric amino acid transporter. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26256001/ · DOI 10.1016/j.biocel.2015.08.004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d085319d-2257-5184-beed-fd4a84af7705","evidence_kind":"source_excerpt","locator":"Lines 66-72","start_line":66,"end_line":72,"excerpt":"## histidine-lat1-subunit\nThe transport pore and its partnering protein have distinct roles.\nPurified human LAT1 alone transported histidine in proteoliposomes; purified CD98 alone did not.\nModel: Recombinant human LAT1 and CD98 reconstitution.\nLimitations: CD98 dispensability for this assay does not mean dispensability for membrane targeting in intact tissues.\nEvidence access: Primary abstract\nLAT1 is the transport competent unit of the LAT1/CD98 heterodimeric amino acid transporter. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26256001/ · DOI 10.1016/j.biocel.2015.08.004","model_system":"Recombinant human LAT1 and CD98 reconstitution.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ce59e6c9-1213-523b-9d0b-400b7a81cd1c","stable_key":"import-911fb3c7-8cc3-5667-b677-5682fab67648","title":"L-Histidine: supply, catabolism, histamine, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"cb672530ec8b4a4542d0f3957e80ca76bb0449e9f988845b0f7681f623c4ec9b","revision_id":"19a7f26c-d9a1-5411-a81d-025f3d98a452","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}