{"id":"81f67e4a-07e4-5d85-a0ad-dcb3967b2590","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-collagen-culture","predicate":"increases_tested","statement":"Increasing medium glycine from the millimolar range increased type-II collagen production in cultured bovine chondrocytes, with larger sustained effects than the tested proline/lysine increments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6f26a39d-7d63-50f8-9e50-5ae2fe3bee72","mechanism_event_label":"Collagen-producing cells responded to extra glycine in a culture assay.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"3dee9fee-d011-5fec-91b8-c36b736af2ff","slug":"bovine-chondrocyte-type-ii-collagen","display_name":"Type-II collagen production by bovine articular chondrocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6f26a39d-7d63-50f8-9e50-5ae2fe3bee72","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-collagen-culture-event","event_type":"observed_relationship","label":"Collagen-producing cells responded to extra glycine in a culture assay.","description":"Increasing medium glycine from the millimolar range increased type-II collagen production in cultured bovine chondrocytes, with larger sustained effects than the tested proline/lysine increments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3dee9fee-d011-5fec-91b8-c36b736af2ff","slug":"bovine-chondrocyte-type-ii-collagen","display_name":"Type-II collagen production by bovine articular chondrocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bovine articular chondrocyte monolayers; 15-day concentration series and collagen ELISA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The paper proposes dietary implications, but the experiment does not establish human glycine essentiality, osteoarthritis causation or cartilage regeneration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Collagen-producing cells responded to extra glycine in a culture assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"High glycine concentration increases collagen synthesis by articular chondrocytes in vitro: acute glycine deficiency could be an important cause of osteoarthritis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30006659/ · DOI 10.1007/s00726-018-2611-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3d605d21-8b3b-5fe1-8284-741851a02136","evidence_kind":"source_excerpt","locator":"Lines 402-408","start_line":402,"end_line":408,"excerpt":"## glycine-collagen-culture\nCollagen-producing cells responded to extra glycine in a culture assay.\nIncreasing medium glycine from the millimolar range increased type-II collagen production in cultured bovine chondrocytes, with larger sustained effects than the tested proline/lysine increments.\nModel: Bovine articular chondrocyte monolayers; 15-day concentration series and collagen ELISA.\nLimitations: The paper proposes dietary implications, but the experiment does not establish human glycine essentiality, osteoarthritis causation or cartilage regeneration.\nEvidence access: Primary full text\nHigh glycine concentration increases collagen synthesis by articular chondrocytes in vitro: acute glycine deficiency could be an important cause of osteoarthritis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30006659/ · DOI 10.1007/s00726-018-2611-x","model_system":"Bovine articular chondrocyte monolayers; 15-day concentration series and collagen ELISA.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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