{"id":"81ea0981-798a-5292-9d12-324cba3650b5","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-mast-cell-augmentation","predicate":"augments_ionophore_response","statement":"Tartrazine at 0.1-1 mM augmented A23187-triggered histamine release in the rat mast-cell assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4909b5e3-899d-5609-a415-33abe47caf2d","mechanism_event_label":"Tartrazine at 0.1-1 mM augmented A23187-triggered histamine release in the rat mast-cell assay.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"fc75ccd2-f6e4-5596-b166-582fc6a7b4f1","slug":"rat-peritoneal-mast-cell-histamine-release","display_name":"Histamine release from rat peritoneal mast cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4909b5e3-899d-5609-a415-33abe47caf2d","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-mast-cell-augmentation-event","event_type":"observed_relationship","label":"Tartrazine at 0.1-1 mM augmented A23187-triggered histamine release in the rat mast-cell assay.","description":"Tartrazine at 0.1-1 mM augmented A23187-triggered histamine release in the rat mast-cell assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fc75ccd2-f6e4-5596-b166-582fc6a7b4f1","slug":"rat-peritoneal-mast-cell-histamine-release","display_name":"Histamine release from rat peritoneal mast cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5e0f278e-3d1a-5725-bbfc-d90ea6c72d26","slug":"calcimycin","display_name":"Calcimycin / calcium ionophore A23187","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"847daefd-ce13-541a-b426-919975168a3d","slug":"histamine","display_name":"Histamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 0.1-1 mM with calcium ionophore A23187","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Maximum augmentation with 0-5 min preincubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified rat peritoneal mast cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The ionophore interaction cannot be generalized to every mast-cell stimulus; higher concentrations had a small inhibitory effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Purified rat peritoneal mast cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine at 0.1-1 mM augmented A23187-triggered histamine release in the rat mast-cell assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The effect of tartrazine on histamine release from rat peritoneal mast cells. (1984). https://pubmed.ncbi.nlm.nih.gov/6204951/ DOI: 10.1016/0192-0561(84)90021-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro preincubation and challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Calcium-ionophore-triggered histamine release","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f30663df-0cd6-5856-b89f-a7d56562e2fa","evidence_kind":"source_excerpt","locator":"Lines 468-477","start_line":468,"end_line":477,"excerpt":"## tartrazine-mast-cell-augmentation\nTartrazine at 0.1-1 mM augmented A23187-triggered histamine release in the rat mast-cell assay.\nModel/species: Purified rat peritoneal mast cells\nTissue: Calcium-ionophore-triggered histamine release\nExposure: Tartrazine 0.1-1 mM with calcium ionophore A23187\nRoute: In vitro preincubation and challenge\nDuration: Maximum augmentation with 0-5 min preincubation\nLimits: The ionophore interaction cannot be generalized to every mast-cell stimulus; higher concentrations had a small inhibitory effect.\nPrimary reference: The effect of tartrazine on histamine release from rat peritoneal mast cells. (1984). https://pubmed.ncbi.nlm.nih.gov/6204951/ DOI: 10.1016/0192-0561(84)90021-3\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Purified rat peritoneal mast cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}