{"id":"81e0f74b-43e8-5155-a818-dbb41f09790e","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a5-not-ergo","predicate":"does_not_transport","statement":"Wild-type human SLC22A5 did not transport ergothioneine in the comparative expression assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"d3a58f8a-4cdc-5455-8446-2bfa7373dcd0","mechanism_event_label":"The main carnitine carrier cannot be assumed to carry ergothioneine.","subject":{"id":"fb9ae9da-e4d3-5557-baac-d8b00e98ba10","slug":"slc22a5","display_name":"Human carnitine transporter OCTN2 / SLC22A5","entity_type_key":"protein"},"object":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"d3a58f8a-4cdc-5455-8446-2bfa7373dcd0","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a5-not-ergo-event","event_type":"observed_relationship","label":"The main carnitine carrier cannot be assumed to carry ergothioneine.","description":"Wild-type human SLC22A5 did not transport ergothioneine in the comparative expression assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fb9ae9da-e4d3-5557-baac-d8b00e98ba10","slug":"slc22a5","display_name":"Human carnitine transporter OCTN2 / SLC22A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human wild-type transporters expressed in HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Related sequence does not establish substrate interchangeability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The main carnitine carrier cannot be assumed to carry ergothioneine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Substrate discrimination by ergothioneine transporter SLC22A4 and carnitine transporter SLC22A5: gain-of-function by interchange of selected amino acids. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19814996/ · DOI 10.1016/j.bbamem.2009.09.019","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2582b11e-298f-5b60-a7bc-ce3b2e9e3faf","evidence_kind":"source_excerpt","locator":"Lines 40-46","start_line":40,"end_line":46,"excerpt":"## ergothioneine-slc22a5-not-ergo\nThe main carnitine carrier cannot be assumed to carry ergothioneine.\nWild-type human SLC22A5 did not transport ergothioneine in the comparative expression assay.\nModel: Human wild-type transporters expressed in HEK293 cells.\nLimitations: Related sequence does not establish substrate interchangeability.\nEvidence access: Primary abstract\nSubstrate discrimination by ergothioneine transporter SLC22A4 and carnitine transporter SLC22A5: gain-of-function by interchange of selected amino acids. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19814996/ · DOI 10.1016/j.bbamem.2009.09.019","model_system":"Human wild-type transporters expressed in HEK293 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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