{"id":"8182c1a1-88e3-5a75-9a5a-892fff64ced2","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-tlr4-loss","predicate":"when_silenced_reduces_tested","statement":"TLR4 silencing reduced cucurbitacin B-induced pyroptosis and associated mitochondrial ROS and calcium signals in human NSCLC cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7034fbe6-8e0a-5610-9b77-aeb1c50b5a97","mechanism_event_label":"Removing the receptor weakened the tested death pathway.","subject":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"object":{"id":"e0adbf00-f459-5826-9a77-d56b09004ad4","slug":"human-cucurbitacin-pyroptosis","display_name":"Human NSCLC pyroptotic response during cucurbitacin B exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7034fbe6-8e0a-5610-9b77-aeb1c50b5a97","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-tlr4-loss-event","event_type":"observed_relationship","label":"Removing the receptor weakened the tested death pathway.","description":"TLR4 silencing reduced cucurbitacin B-induced pyroptosis and associated mitochondrial ROS and calcium signals in human NSCLC cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3f792f3c-3129-5995-8341-2295c5ea5b27","slug":"tlr4","display_name":"Human toll-like receptor 4 / TLR4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e0adbf00-f459-5826-9a77-d56b09004ad4","slug":"human-cucurbitacin-pyroptosis","display_name":"Human NSCLC pyroptotic response during cucurbitacin B exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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