{"id":"816cf546-a6de-5dcf-8c65-9d83d3a804a1","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-human-nis-uptake","predicate":"mediates_uptake","statement":"Expression of cloned human NIS conferred perchlorate-sensitive iodide uptake on COS-7 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6bfe13ea-b51f-53c7-81af-dc9701531f18","mechanism_event_label":"Human NIS can bring iodide into cells.","subject":{"id":"0d051c22-c0d2-5c22-91ad-4ee6894dc7a0","slug":"slc5a5","display_name":"Human sodium/iodide symporter / SLC5A5","entity_type_key":"protein"},"object":{"id":"759b6f24-098d-5b19-b891-a5ad49fade50","slug":"cellular-iodide-uptake","display_name":"Cellular iodide uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6bfe13ea-b51f-53c7-81af-dc9701531f18","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-human-nis-uptake-event","event_type":"biochemical_relationship","label":"Human NIS can bring iodide into cells.","description":"Expression of cloned human NIS conferred perchlorate-sensitive iodide uptake on COS-7 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0d051c22-c0d2-5c22-91ad-4ee6894dc7a0","slug":"slc5a5","display_name":"Human sodium/iodide symporter / SLC5A5","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"759b6f24-098d-5b19-b891-a5ad49fade50","slug":"cellular-iodide-uptake","display_name":"Cellular iodide uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NIS cDNA expressed in COS-7 monkey cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Transient human NIS cDNA expression; iodide and perchlorate concentrations not reported in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Heterologous uptake establishes protein function, not whole-body iodine absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein in African green monkey cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human NIS can bring iodide into cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-human-nis1996] Cloning of the human sodium lodide symporter. (1996). https://pubmed.ncbi.nlm.nih.gov/8806637/ DOI: 10.1006/bbrc.1996.1358","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"COS-7 plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"091f198a-76ed-547a-ac43-7a730e016594","evidence_kind":"source_excerpt","locator":"Lines 154-165","start_line":154,"end_line":165,"excerpt":"### iodine-trans-human-nis-uptake\nExpression of cloned human NIS conferred perchlorate-sensitive iodide uptake on COS-7 cells.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Human NIS can bring iodide into cells.\norganism: Human protein in African green monkey cells\ntissue_or_cell_type: COS-7 plasma membrane\nexperimental_model: Human NIS cDNA expressed in COS-7 monkey cells\nlimitations: Heterologous uptake establishes protein function, not whole-body iodine absorption.\nexposure: Transient human NIS cDNA expression; iodide and perchlorate concentrations not reported in the abstract.\ncross_nutrient: false\n[iodine-trans-human-nis1996] Cloning of the human sodium lodide symporter. (1996). https://pubmed.ncbi.nlm.nih.gov/8806637/ DOI: 10.1006/bbrc.1996.1358","model_system":"Human NIS cDNA expressed in COS-7 monkey cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-trans-human-nis1996] Cloning of the human sodium lodide symporter. (1996). https://pubmed.ncbi.nlm.nih.gov/8806637/ DOI: 10.1006/bbrc.1996.1358","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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