{"id":"811a4e5f-e756-5ed7-91f6-10f6afe00a07","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-dbp-null-retention","predicate":"deletion_reduces","statement":"Deletion of mouse vitamin D-binding protein accelerated the loss of injected radiolabeled calcifediol from plasma.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"41c6a6c2-29c2-5137-8983-ad5ece385d4b","mechanism_event_label":"The blood carrier helps vitamin D precursor stay in circulation.","subject":{"id":"6c1f7a61-aa4b-56da-8c5b-9c5c6e502a67","slug":"mouse-gc-gene","display_name":"Mouse Gc gene","entity_type_key":"gene"},"object":{"id":"2d1e1448-93bb-5c1e-8374-884294bda1f6","slug":"circulating-calcifediol-retention","display_name":"Circulating calcifediol retention","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"41c6a6c2-29c2-5137-8983-ad5ece385d4b","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-dbp-null-retention-event","event_type":"biochemical_relationship","label":"The blood carrier helps vitamin D precursor stay in circulation.","description":"Deletion of mouse vitamin D-binding protein accelerated the loss of injected radiolabeled calcifediol from plasma.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0c858f70-5b07-5db5-8494-bfe3d8733b34","slug":"mouse-gc","display_name":"Mouse vitamin D-binding protein / Gc","entity_type_key":"protein"},"role":"deleted_carrier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"role":"tracer","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6c1f7a61-aa4b-56da-8c5b-9c5c6e502a67","slug":"mouse-gc-gene","display_name":"Mouse Gc gene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2d1e1448-93bb-5c1e-8374-884294bda1f6","slug":"circulating-calcifediol-retention","display_name":"Circulating calcifediol retention","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full primary Methods, Vitamin D kinetics; Results, kinetics of 25(OH)D3; abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiotracer kinetics in Gc-null and wild-type mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"After 4-6 weeks on D3-deficient diet, 0.5 microCi or 100000 cpm tracer in homologous serum was injected intravenously; sampling over 24 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Both genotypes experienced dietary depletion; the comparison isolates an additional carrier defect. 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(1999). https://pubmed.ncbi.nlm.nih.gov/9916136/ DOI: 10.1172/jci5244","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"plasma and liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"acd75e37-7a0c-5ec9-a50c-4b35b3763d74","evidence_kind":"source_excerpt","locator":"Lines 272-285","start_line":272,"end_line":285,"excerpt":"### vd-act-dbp-null-retention\nDeletion of mouse vitamin D-binding protein accelerated the loss of injected radiolabeled calcifediol from plasma.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The blood carrier helps vitamin D precursor stay in circulation.\norganism: Mus musculus\ntissue_or_cell_type: plasma and liver\nexperimental_model: Radiotracer kinetics in Gc-null and wild-type mice\nlimitations: Both genotypes experienced dietary depletion; the comparison isolates an additional carrier defect. Tracer plasma residence is not a human dietary-dose trial.\nexposure: After 4-6 weeks on D3-deficient diet, 0.5 microCi or 100000 cpm tracer in homologous serum was injected intravenously; sampling over 24 h.\ncross_nutrient: false\nevidence_location: Full primary Methods, Vitamin D kinetics; Results, kinetics of 25(OH)D3; abstract.\nnutrient: Vitamin D2 and D3\n[safadi1999] Osteopathy and resistance to vitamin D toxicity in mice null for vitamin D binding protein. (1999). https://pubmed.ncbi.nlm.nih.gov/9916136/ DOI: 10.1172/jci5244","model_system":"Radiotracer kinetics in Gc-null and wild-type mice","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [safadi1999] Osteopathy and resistance to vitamin D toxicity in mice null for vitamin D binding protein. 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