{"id":"81128922-25ee-5538-8c8b-e7b574238e47","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-severe-marrow-megaloblastosis","predicate":"prolonged_restriction_preceded_megaloblastic_changes","statement":"Marrow examination at day 575 confirmed megaloblastic anemia; blood counts recovered during the subsequent replete-diet/supplement phase.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a11af999-3ff4-5fa6-b1c0-0514df8efb80","mechanism_event_label":"The shortage eventually coincided with abnormal marrow-cell maturation and reversible blood-count changes.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"32338c0b-bc3c-56f8-9228-f82b3be962ee","slug":"megaloblastic-anemia","display_name":"Megaloblastic anemia","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a11af999-3ff4-5fa6-b1c0-0514df8efb80","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-severe-marrow-megaloblastosis-event","event_type":"observed_intervention","label":"The shortage eventually coincided with abnormal marrow-cell maturation and reversible blood-count changes.","description":"Marrow examination at day 575 confirmed megaloblastic anemia; blood counts recovered during the subsequent replete-diet/supplement phase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32338c0b-bc3c-56f8-9228-f82b3be962ee","slug":"megaloblastic-anemia","display_name":"Megaloblastic anemia","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Single initially folate-replete 58-year-old male self-experiment with serial blood assays and marrow examinations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Prolonged severe folate restriction, major energy restriction/weight loss, and multiple other nutrient supplements; replete diet plus folic acid during recovery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Major energy restriction and weight loss confound isolated nutrient attribution. Other nutrients were supplemented; neither liver folate content nor a unique recovery dose was directly established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The shortage eventually coincided with abnormal marrow-cell maturation and reversible blood-count changes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fol-golding2014] Severe experimental folate deficiency in a human subject - a longitudinal study of biochemical and haematological responses as megaloblastic anaemia develops (2014). https://pubmed.ncbi.nlm.nih.gov/25332850/ DOI: 10.1186/2193-1801-3-442","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human bone marrow and peripheral blood","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"243d28cc-86d5-5326-a00d-ffc0980d8d1f","evidence_kind":"source_excerpt","locator":"Lines 1529-1539","start_line":1529,"end_line":1539,"excerpt":"### fol-severe-marrow-megaloblastosis\nMarrow examination at day 575 confirmed megaloblastic anemia; blood counts recovered during the subsequent replete-diet/supplement phase.\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The shortage eventually coincided with abnormal marrow-cell maturation and reversible blood-count changes.\norganism: Homo sapiens\ntissue_or_cell_type: Human bone marrow and peripheral blood\nexperimental_model: Single initially folate-replete 58-year-old male self-experiment with serial blood assays and marrow examinations.\nlimitations: Major energy restriction and weight loss confound isolated nutrient attribution. Other nutrients were supplemented; neither liver folate content nor a unique recovery dose was directly established.\nexposure: Prolonged severe folate restriction, major energy restriction/weight loss, and multiple other nutrient supplements; replete diet plus folic acid during recovery.\n[fol-golding2014] Severe experimental folate deficiency in a human subject - a longitudinal study of biochemical and haematological responses as megaloblastic anaemia develops (2014). https://pubmed.ncbi.nlm.nih.gov/25332850/ DOI: 10.1186/2193-1801-3-442","model_system":"Single initially folate-replete 58-year-old male self-experiment with serial blood assays and marrow examinations.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fol-golding2014] Severe experimental folate deficiency in a human subject - a longitudinal study of biochemical and haematological responses as megaloblastic anaemia develops (2014). https://pubmed.ncbi.nlm.nih.gov/25332850/ DOI: 10.1186/2193-1801-3-442","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}