{"id":"809a0ac4-0d3b-5452-a894-d6ee57c998f1","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-lactisole-receptor","predicate":"inhibits_sucrose_response","statement":"Lactisole inhibited the sucrose-evoked response of expressed human TAS1R2/TAS1R3.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"14cd1f93-4f41-52d3-bbe6-ea99417873fe","mechanism_event_label":"Lactisole inhibited the sucrose-evoked response of expressed human TAS1R2/TAS1R3.","subject":{"id":"7ccdbbaf-dfdc-5e35-bcd7-a360b0db78e1","slug":"lactisole","display_name":"Lactisole","entity_type_key":"small_molecule"},"object":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"14cd1f93-4f41-52d3-bbe6-ea99417873fe","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-lactisole-receptor-event","event_type":"observed_relationship","label":"Lactisole inhibited the sucrose-evoked response of expressed human TAS1R2/TAS1R3.","description":"Lactisole inhibited the sucrose-evoked response of expressed human TAS1R2/TAS1R3.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7ccdbbaf-dfdc-5e35-bcd7-a360b0db78e1","slug":"lactisole","display_name":"Lactisole","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"300 mM sucrose with or without 1.25 mM lactisole","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute calcium response","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Selected primary full-text sections inspected via indexed PMC page; local XML has abstract only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Human receptor in an expression system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered coupling and high assay concentration do not measure human dietary absorption. Rat lactisole sensitivity differs. Selected full-text sections inspected; archived XML is abstract only.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lactisole inhibited the sucrose-evoked response of expressed human TAS1R2/TAS1R3.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human receptors for sweet and umami taste. (2002). https://pubmed.ncbi.nlm.nih.gov/11917125/ DOI: 10.1073/pnas.072090199","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro receptor stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Recombinant sweet-receptor calcium-response assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"50a5e11d-2269-5779-847a-2637420febed","evidence_kind":"source_excerpt","locator":"Lines 67-77","start_line":67,"end_line":77,"excerpt":"## sucrose-lactisole-receptor\nLactisole inhibited the sucrose-evoked response of expressed human TAS1R2/TAS1R3.\nModel/species: Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells\nTissue: Recombinant sweet-receptor calcium-response assay\nExposure: 300 mM sucrose with or without 1.25 mM lactisole\nRoute: In vitro receptor stimulation\nDuration: Acute calcium response\nExposure scope: Human receptor in an expression system\nLimits: Engineered coupling and high assay concentration do not measure human dietary absorption. Rat lactisole sensitivity differs. Selected full-text sections inspected; archived XML is abstract only.\nReference: Human receptors for sweet and umami taste. (2002). https://pubmed.ncbi.nlm.nih.gov/11917125/ DOI: 10.1073/pnas.072090199\nAccess: Selected primary full-text sections inspected via indexed PMC page; local XML has abstract only.","model_system":"Human TAS1R2/TAS1R3 coexpressed with engineered G-alpha15 in HEK-derived cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. Not publisher full text.","file_path":"","sha256":"5e68ccbade7a80fc03778495756f0eeeeba5038cd10d5bef8e33cb1e93e4b044","revision_id":"6edb8ffc-11c5-5cd9-852c-85adb71165a8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}