{"id":"807d7756-9c4f-5937-904d-03df82d51abd","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-got1-reaction","predicate":"catalyzes-transamination-of","statement":"Human GOT1 catalyzes reversible amino transfer between aspartate and 2-oxoglutarate, producing oxaloacetate and glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"372da90a-1558-5637-bce1-7d5d8ada2a1c","mechanism_event_label":"This B6-dependent enzyme links amino-acid and carbon metabolism.","subject":{"id":"4c4eee53-67f4-5c46-aa5f-ba2bcaf3424d","slug":"got1","display_name":"Human cytosolic aspartate aminotransferase / GOT1","entity_type_key":"protein"},"object":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"372da90a-1558-5637-bce1-7d5d8ada2a1c","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-got1-reaction-event","event_type":"biochemical_relationship","label":"This B6-dependent enzyme links amino-acid and carbon metabolism.","description":"Human GOT1 catalyzes reversible amino transfer between aspartate and 2-oxoglutarate, producing oxaloacetate and glutamate.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"4c4eee53-67f4-5c46-aa5f-ba2bcaf3424d","slug":"got1","display_name":"Human cytosolic aspartate aminotransferase / GOT1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"32119d9f-54be-5e6d-98da-1621b0e2421d","slug":"oxaloacetate","display_name":"Oxaloacetate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified human cytosolic GOT1 and GPT; coupled kinetic assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Kinetic assays at pH 7.4 and 37 C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme evidence does not define dietary requirements or cellular PLP thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This B6-dependent enzyme links amino-acid and carbon metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-transaminases-2022] Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases. (2022). https://pmc.ncbi.nlm.nih.gov/articles/PMC8875635/ DOI: 10.3390/molecules27041398","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ab6e71a5-cf21-574b-8e3a-f27b50345489","evidence_kind":"source_excerpt","locator":"Lines 741-751","start_line":741,"end_line":751,"excerpt":"### b6-met-got1-reaction\nHuman GOT1 catalyzes reversible amino transfer between aspartate and 2-oxoglutarate, producing oxaloacetate and glutamate.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This B6-dependent enzyme links amino-acid and carbon metabolism.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Purified human cytosolic GOT1 and GPT; coupled kinetic assays\nlimitations: Purified-enzyme evidence does not define dietary requirements or cellular PLP thresholds.\nexposure: Kinetic assays at pH 7.4 and 37 C\n[b6-transaminases-2022] Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases. (2022). https://pmc.ncbi.nlm.nih.gov/articles/PMC8875635/ DOI: 10.3390/molecules27041398","model_system":"Purified human cytosolic GOT1 and GPT; coupled kinetic assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-transaminases-2022] Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases. (2022). https://pmc.ncbi.nlm.nih.gov/articles/PMC8875635/ DOI: 10.3390/molecules27041398","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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