{"id":"8062ea8f-a726-5a50-9ce9-0aedc10325d0","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-extracellular-gsh-loss","predicate":"causes_tested","statement":"Extracellular glutamate exposure lowered glutathione and increased intracellular peroxides in N18-RE-105 cells alongside inhibited cystine uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"89e2179a-0942-5c03-9927-74ccc37ddac7","mechanism_event_label":"Oxidative injury arose through precursor supply, a different route from opening an excitatory receptor.","subject":{"id":"dae8bb49-c09a-54a5-b885-dcf63894e53c","slug":"n18re105-extracellular-glutamate-exposure","display_name":"Extracellular glutamate challenge in N18-RE-105 cells","entity_type_key":"cellular_process"},"object":{"id":"92eb7859-30ee-56c5-bcd4-de680a7eace2","slug":"n18re105-oxidative-injury","display_name":"Oxidative injury in N18-RE-105 neuronal hybrid cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"89e2179a-0942-5c03-9927-74ccc37ddac7","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-extracellular-gsh-loss-event","event_type":"observed_relationship","label":"Oxidative injury arose through precursor supply, a different route from opening an excitatory receptor.","description":"Extracellular glutamate exposure lowered glutathione and increased intracellular peroxides in N18-RE-105 cells alongside inhibited cystine uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dae8bb49-c09a-54a5-b885-dcf63894e53c","slug":"n18re105-extracellular-glutamate-exposure","display_name":"Extracellular glutamate challenge in N18-RE-105 cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"92eb7859-30ee-56c5-bcd4-de680a7eace2","slug":"n18re105-oxidative-injury","display_name":"Oxidative injury in N18-RE-105 neuronal hybrid cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"4fdc07ad-8e1d-56e6-a03c-a8505891a79a","slug":"n18re105-cystine-uptake","display_name":"Cystine uptake in N18-RE-105 neuronal hybrid cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Neuronal hybrid-cell transport, glutathione and peroxide assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A specific experimental toxicity mechanism is retained without inferring normal food-related brain injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; 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