{"id":"80379410-e01f-58bd-aeae-fcd3c6da96e6","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-pf-chrebp","predicate":"reduces_fructose_activation","statement":"PF-06835919 reduced fructose-associated nuclear ChREBP localization in primary rat hepatocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"83880803-c609-5bec-b150-cee24dba89c8","mechanism_event_label":"PF-06835919 reduced fructose-associated nuclear ChREBP localization in primary rat hepatocytes.","subject":{"id":"2bddbb8e-14f7-5d4d-a89e-65ed95a2b3da","slug":"pf-06835919","display_name":"PF-06835919","entity_type_key":"drug"},"object":{"id":"b8f66bfe-44d9-529e-acbb-2156b74db074","slug":"rat-mlxipl","display_name":"Rat carbohydrate-responsive element-binding protein / ChREBP","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"83880803-c609-5bec-b150-cee24dba89c8","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-pf-chrebp-event","event_type":"observed_relationship","label":"PF-06835919 reduced fructose-associated nuclear ChREBP localization in primary rat hepatocytes.","description":"PF-06835919 reduced fructose-associated nuclear ChREBP localization in primary rat hepatocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2bddbb8e-14f7-5d4d-a89e-65ed95a2b3da","slug":"pf-06835919","display_name":"PF-06835919","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b8f66bfe-44d9-529e-acbb-2156b74db074","slug":"rat-mlxipl","display_name":"Rat carbohydrate-responsive element-binding protein / ChREBP","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3c379840-5688-5b5d-84c0-5950b4fd8f17","slug":"fructose","display_name":"Fructose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"10 mM fructose with or without 30 micromolar PF-06835919","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Overnight","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata inspected.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary rat hepatocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Isolated fructose / investigational drug","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-model transcriptional effect; not established human lipid or disease prevention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"HFCS chapter: actual formulation studies, component biochemistry and interventions are explicitly distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"high-fructose-corn-syrup","display_name":"High-Fructose Corn Syrup / HFCS","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Primary rat hepatocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PF-06835919 reduced fructose-associated nuclear ChREBP localization in primary rat hepatocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pharmacologic inhibition of ketohexokinase prevents fructose-induced metabolic dysfunction. 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